Characterization of gut microbial and metabolite alterations in faeces of Goto Kakizaki rats using metagenomic and untargeted metabolomic approach  

作　　者：Jin-Dong Zhao Min Sun Yan Li Chan-Juan Yu Ruo-Dong Cheng Si-Hai Wang Xue Du Zhao-Hui Fang 

机构地区：[1]Department of Endocrinology,The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,Anhui Province,China [2]Graduate School,Anhui University of Chinese Medicine,Hefei 230012,Anhui Province,China [3]School of Life Sciences,Anhui University,Hefei 230039,Anhui Province,China [4]Department of Infectious Disease,The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,Anhui Province,China

出　　处：《World Journal of Diabetes》2023年第3期255-270,共16页世界糖尿病杂志（英文版）（电子版）

基　　金：Supported by the University Scientific Research Projects of Anhui,No. KJ2020A0401 and 2022AH050491;the open fund of the Ministry of Education Key Laboratory of Glucolipid Metabolic Disorder,No. GYDKFXM01;the Anhui University Collaborative Innovation Project,No. GXXT-2020-025;the National Natural Science Foundation of China,No. 82174153;the National Key Research and Development Program,No. 2018YFC1704202;the Anhui Provincial Quality Engineering Project of Universities,No. 2021jyxm0834;the Major and Difficult Diseases Project of Anhui Province,No. 2021zdynjb06;the Clinical Research Project of Anhui University of Traditional Chinese Medicine,No. 2021yfylc01

摘　　要：BACKGROUND In recent years,the incidence of type 2 diabetes(T2DM)has shown a rapid growth trend.Goto Kakizaki(GK)rats are a valuable model for the study of T2DM and share common glucose metabolism features with human T2DM patients.A series of studies have indicated that T2DM is associated with the gut microbiota composition and gut metabolites.We aimed to systematically characterize the faecal gut microbes and metabolites of GK rats and analyse the relationship between glucose and insulin resistance.AIM To evaluate the gut microbial and metabolite alterations in GK rat faeces based on metagenomics and untargeted metabolomics.METHODS Ten GK rats(model group)and Wistar rats(control group)were observed for 10 wk,and various glucose-related indexes,mainly including weight,fasting blood glucose(FBG)and insulin levels,homeostasis model assessment of insulin resistance(HOMA-IR)and homeostasis model assessment ofβcell(HOMA-β)were assessed.The faecal gut microbiota was sequenced by metagenomics,and faecal metabolites were analysed by untargeted metabolomics.Multiple metabolic pathways were evaluated based on the differential metabolites identified,and the correlations between blood glucose and the gut microbiota and metabolites were analysed.RESULTS The model group displayed significant differences in weight,FBG and insulin levels,HOMA-IR and HOMA-βindexes(P<0.05,P<0.01)and a shift in the gut microbiota structure compared with the control group.The results demonstrated significantly decreased abundances of Prevotella sp.CAG:604 and Lactobacillus murinus(P<0.05)and a significantly increased abundance of Allobaculum stercoricanis(P<0.01)in the model group.A correlation analysis indicated that FBG and HOMA-IR were positively correlated with Allobaculum stercoricanis and negatively correlated with Lactobacillus murinus.An orthogonal partial least squares discriminant analysis suggested that the faecal metabolic profiles differed between the model and control groups.Fourteen potential metabolic biomarkers,including glycoche

关 键 词：Type 2 diabetes mellitus Gut microbial Metabolites Goto-Kakizaki rats METAGENOMICS Untargeted metabolomics 

分 类 号：R587.1[医药卫生—内分泌]