Temporal pattern of humoral immune response in mild cases of COVID-19  

作　　者：Isadora Maria Pilati Campos Milena Marques Gabrielle Caroline Peiter Ana Paula Carneiro Brandalize Mauricio Bedim dos Santos Fabrício Freire de Melo Kádima Nayara Teixeira 

机构地区：[1]Campus Toledo,Universidade Federal do Paraná,Toledo 85.919-899,Paraná,Brazil [2]Setor Palotina,Universidade Federal do Paraná,Palotina 85.950-000,Paraná,Brazil [3]Campus Anísio Teixeira,Universidade Federal da Bahia,Vitória da Conquista 45029-094,Bahia,Brazil

出　　处：《World Journal of Biological Chemistry》2023年第2期40-51,共12页世界生物化学杂志（英文版）（电子版）

摘　　要：BACKGROUND Understanding the humoral response pattern of coronavirus disease 2019(COVID-19)is one of the essential factors to better characterize the immune memory of patients,which allows understanding the temporality of reinfection,provides answers about the efficacy and durability of protection against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),and consequently helps in global public health and vaccination strategy.Among the patients who became infected with SARS-CoV-2,the majority who did not progress to death were those who developed the mild COVID-19,so understanding the pattern and temporality of the antibody response of these patients is certainly relevant.AIM To investigate the temporal pattern of humoral response of specific immunoglobulin G(IgG)in mild cases of COVID-19.METHODS Blood samples from 191 COVID-19 real-time reverse transcriptase-polymerase chain reaction(RT-qPCR)-positive volunteers from the municipality of Toledo/Paraná/Brazil,underwent two distinct serological tests,enzyme-linked immunosorbent assay,and detection of anti-nucleocapsid IgG.Blood samples and clinicoepidemiological data of the volunteers were collected between November 2020 and February 2021.All assays were performed in duplicate and the manufacturers'recommendations were strictly followed.The data were statistically analyzed using multiple logistic regression;the variables were selected by applying the P<0.05 criterion.RESULTS Serological tests to detect specific IgG were performed on serum samples from volunteers who were diagnosed as being positive by RT-qPCR for COVID-19 or had disease onset in the time interval from less than 1 mo to 7 mo.The time periods when the highest number of participants with detectable IgG was observed were 1,2 and 3 mo.It was observed that 9.42%of participants no longer had detectable IgG antibodies 1 mo only after being infected with SARS-CoV-2 and 1.57%were also IgG negative at less than 1 mo.At 5 mo,3.14%of volunteers were IgG negative,and at 6 or 7 mo,1 volunteer(0.52%)had

关 键 词：Humoral response Immunoglobulin G antibody Immune memory Mild cases COVID-19 SARS-CoV-2 infection Serological test 

分 类 号：R511[医药卫生—内科学]