蛋白质折叠与力学传感的单分子磁镊操纵研究进展  

Progress of single-molecule magnetic-tweezers manipulation study on protein folding and force-sensing mechanisms

作　　者：乐世敏 陈虎 LE Shimin;CHEN Hu(Fujian Provincial Key Lab for Soft Functional Materials Research,Research Institute for Biomimetics and Soft Matter,College of Physical Science and Technology,Xiamen University,Xiamen 361005,China)

机构地区：[1]厦门大学物理科学与技术学院,生物仿生与软物质研究院,福建省柔性功能材料重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2023年第2期269-279,共11页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(11874309,12174322,32271367)。

摘　　要：蛋白质的折叠与相互作用是物理、生物等多学科交叉领域关注的基本问题.力生物学的前沿研究表明,一系列对力敏感的蛋白质在机械力作用下的去折叠与复折叠动力学及相互作用调控,是实现其力感知的物理机制.前沿单分子操纵技术的发展使得在单分子水平定量探究蛋白质的折叠与细胞力学传感的分子机制成为可能.本文重点介绍近年来蛋白质折叠与力学传感的单分子磁镊操纵研究进展,包括单结构域蛋白质的折叠-去折叠动力学、自由能曲面的构造,及细胞黏着斑与胞间连接的力敏感蛋白行使生物功能的分子机制.Protein folding and interaction are basic problems of interdisciplinary field between physics and biology.Most recent studies on mechanobiology have identified the regulation of the folding/unfolding dynamics and interactions of force-sensitive proteins by mechanical force as the physical mechanism of force sensing.Besides,advanced single-molecule manipulation techniques can possibly reveal the molecular mechanism of protein folding and cellular force sensing quantitatively at single-molecule level.In this review,we mainly introduces the achievements of recent years on single-molecule magnetic-tweezers manipulation study of protein folding and force sensing,including the folding and unfolding dynamics,construction of free energy landscape of single-domain proteins,and molecular mechanisms of the force-sensitive proteins at the focal adhesion and cell-cell junctions for performing their biological functions.

关 键 词：蛋白质折叠 蛋白质相互作用 力生物学 力敏感蛋白 单分子操纵 磁镊 

分 类 号：Q615[生物学—生物物理学]