基于转录组和蛋白组数据联合分析浅光休眠烟草种子暗萌发的分子网络  

Analysis of molecular networks for dark germination of shallow photodormant Nicotiana tabacum seeds based on transcriptomic and proteomic data

作　　者：刘一灵 刘奇源 董帅 张民 罗燕 李振华 LIU Yiling;LIU Qiyuan;DONG Shuai;ZHANG Min;LUO Yan;LI Zhenhua(CollegeofTobacco,GuizhouUniversity,Guiyang550025,Guizhou,China;CollegeofAgriculture,Guizhou University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州大学烟草学院,贵州贵阳550025 [2]贵州大学农学院,贵州贵阳550025

出　　处：《浙江农林大学学报》2023年第2期237-243,共7页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(32060512,31860420);贵州省烟草公司资助项目(201904)。

摘　　要：【目的】旨在挖掘浅光休眠烟草Nicotiana tabacum种子在黑暗下的调控基因和分子网络。【方法】以浅光休眠烟草Y85种子为实验材料,通过整合转录组和蛋白组数据,挖掘其暗萌发的分子网络。【结果】胚根突出前后蛋白和(或)mRNA表达差异存在5种差异类型,其中共同上调表达的蛋白(基因)有BoGH3B、MAN1、ERD3、MLP31、FAP1等,共同下调表达的蛋白(基因)有NEC1、MFT、ECP63、SOP1、LE25、SBP65等。上述差异蛋白(基因)富集的信号通路包括果糖和甘露糖代谢、甘露聚糖分解、内甘露聚糖-1,4-β-甘露糖苷酶活性;而发生功能的细胞成分包括线粒体、膜组成部分和叶绿体。【结论】通过整合转录组和蛋白组数据初步构建了浅光休眠烟草Y85种子的暗萌发调控网络。[Objective]This study aims to explore the regulatory genes and molecular network for shallow photodormant Nicotiana tabacum in the dark.[Method]Shallow photodormant N.tabacum Y85 seeds were used as experimental materials,and the molecular network of dark germination was studied by integrating transcriptome and proteome data.[Result]There were five types of differences in the expression of protein or mRNA expression before and after radicle protrusion,among which the jointly up-regulated proteins(genes)were BoGH3B,MAN1,ERD3,MLP31,FAP1,etc.,and the jointly down-regulated proteins(genes)were NEC1,MFT,ECP63,SOP1,LE25,SBP65,and so on.The signal pathways enriched by the above proteins(genes)included fructose and mannose metabolism,mannan decomposition,endomannan-1,4-β-mannosidase activity.Functional cellular components included mitochondria,membrane components and chloroplasts.[Conclusion]By integrating transcriptomic and proteomic data,the dark germination regulatory network for shallow photodormant Y85 seeds was preliminarily constructed.

关 键 词：烟草 光休眠种子 暗萌发 转录组 蛋白组 联合分析 

分 类 号：S330.2[农业科学—作物遗传育种]