紫薇LiCMB1基因的克隆及表达特性分析  

Cloning and expression characteristics of LiCMB1 gene in Lagerstroemia indica

作　　者：尚林雪 王群 张国哲 赵雨 顾翠花[1,2,3] SHANG Linxue;WANG Qun;ZHANG Guozhe;ZHAO Yu;GU Cuihua(College of Landscape and Architecture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Zhejiang Province Key Laboratory of Germplasm Innovation and Utilization for Garden Plants,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学风景园林与建筑学院,浙江杭州311300 [2]浙江农林大学浙江省园林植物种质创新与利用重点实验室,浙江杭州311300 [3]浙江农林大学南方园林植物种质创新与利用国家林业和草原局重点实验室,浙江杭州311300

出　　处：《浙江农林大学学报》2023年第2期330-337,共8页Journal of Zhejiang A&F University

基　　金：浙江省自然科学基金资助项目(LY21C160001);浙江省农业新品种重大专项花卉育种专项(2021C02071-4)。

摘　　要：【目的】克隆紫薇Lagerstroemia indica LiCMB1基因并分析其在紫薇花芽分化的不同时期及不同组织和器官中的表达,探讨LiCMB1基因的表达特性。【方法】利用简单克隆技术从紫薇中克隆得到LiCMB1的基因序列,通过ExPasy等在线工具对其进行蛋白质理化性质分析,使用MEGA 6.0构建系统进化树,结合紫薇花芽分化的表型观察和石蜡切片,采用实时荧光定量PCR分析花芽分化的不同时期及不同组织和器官中LiCMB1基因的表达。【结果】LiCMB1基因属于MADS-box家族SEP类基因,除了具有典型的MADS_MEF2_like和K-box结构域外,靠近C端处还含有一个SEP motif保守基序;LiCMB1在紫薇花芽分化过程中呈现先上升后下降的表达趋势,在各组织和器官中均有表达,表达量从高到低依次为雌蕊、萼片、芽、长雄蕊、短雄蕊、花瓣、叶、茎、根,说明LiCMB1可能对紫薇的花芽分化起到重要作用,且参与调控花器官发育。【结论】LiCMB1基因属于MADS-box家族的SEP基因,在紫薇花芽分化的前期发挥重要作用,尤其是在花萼分化期表达量最高,组织特异性分析表明该基因很可能参与了调控花器官发育。[Objective]The LiCMB1 gene of Lagerstroemia indica was cloned,and its expressions in different stages of flower bud differentiation and different tissues and organs were analyzed,so as to explore the expression characteristics of LiCMB1 gene.[Method]The gene sequence of LiCMB1 was cloned from L.indica by simple cloning technology.Physical and chemical properties of the protein were analyzed by online tools including ExPasy,and phylogenetic tree was constructed by MEGA 6.0 software.Combined with the phenotypic observation and paraffin section of L.indica flower bud differentiation,the expressions of LiCMB1 gene in different stages of flower bud differentiation and different tissues and organs were analyzed by real-time quantitative PCR(RT-qPCR).[Result]LiCMB1 gene belongs to SEP gene of MADS-box family,except for typical MADS_MEF2_like and K-box structure domain,there is also a SEP motif conserved motif near the Cend.The results of RT-qPCR showed that the expression trend of LiCMB1 increased first and then decreased in the process of flower bud differentiation of L.indica.It is expressed in different tissues and organs,and the expression levels of LiCMB1 from high to low were in the order of pistil,sepal,bud,long stamen,short stamen,petal,leaf,stem,root,indicating that LiCMB1 may play an important role in flower bud differentiation and participate in the regulation of flower organ development.[Conclusion]LiCMB1 gene belongs to the SEP gene of MADS-box family.It plays an important role in the early stage of flower bud differentiation of L.indica,especially in the calyx differentiation period.Tissue specificity analysis indicated that it was likely involved in the regulation of floral organ development.

关 键 词：紫薇 LiCMB1基因 花芽分化 花器官发育 表达分析 

分 类 号：Q78[生物学—分子生物学] S685[农业科学—观赏园艺]