针刺通过Wnt信号通路对绝经后骨质疏松骨折大鼠骨密度和骨代谢的作用研究  

Effect of acupuncture on bone mineral density and bone metabolism in postmenopausal osteoporotic fracture rats through Wnt signaling pathway

作　　者：郭迪 高志攀 张亚平 吴丹[2] GUO Di;GAO Zhipan;ZHANG Yaping;WU Dan(Department of Rehabilitation,Department of Treatment and Prevention of Diseases,Puyang Hospital of Traditional Chinese Medicine,Puyang 457003,China;Department of Osteoporosis,Luoyang Orthopedic Hospital,Zhengzhou 450000,China)

机构地区：[1]河南省濮阳市中医医院康复一科,河南濮阳457003 [2]河南省洛阳正骨医院骨质疏松科,河南郑州450000

出　　处：《中国骨质疏松杂志》2023年第3期349-355,384,共8页Chinese Journal of Osteoporosis

基　　金：河南省中医药科学研究专项课题(2019ZY2084)。

摘　　要：目的探讨针刺对绝经后骨质疏松骨折大鼠骨损伤的治疗作用及其可能作用机制。方法选取36只雌性SD大鼠采用双侧卵巢摘除法建立绝经后骨质疏松症模型,造模成功3周后采用闭合骨折法建立股骨骨折大鼠模型,随机分为模型组、针刺组和针刺+Wnt拮抗剂组,另设假手术组。针刺组给予针刺治疗,针刺+Wnt拮抗剂组给予针刺治疗同时皮下注射20 mg/kg Wnt拮抗剂DKK1;假手术组及模型组仅给予常规饲养。检测各组大鼠股骨骨密度(BMD)、组织矿物质密度(TMD)、骨体积分数(BV/TV)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th)和骨小梁分离度(Tb.Sp)、血清炎性因子和成骨影响因子水平;HE染色观察股骨组织病理学变化,评定骨损伤情况;RT-PCR和Western blot检测大鼠骨痂组织分泌型蛋白Wnt3a和β连环蛋白(β-catenin)mRNA与蛋白表达情况。结果与假手术组比较,模型组大鼠ALP、PINP、股骨组织病理学评分、BMD、TMD、BV/TV、Tb.N、Tb.Th、Wnt3a和β-catenin mRNA与蛋白表达水平降低,Tb.Sp、TNF-α、IL-1β和IL-6水平升高(P<0.05)。与模型组比较,针刺组大鼠ALP、PINP、股骨组织病理学评分、BMD、TMD、BV/TV、Tb.N、Tb.Th、Wnt3a和β-catenin mRNA与蛋白表达水平升高,Tb.Sp、TNF-α、IL-1β和IL-6水平降低(P<0.05)。与针刺组比较,针刺+Wnt拮抗剂组大鼠ALP、PINP、股骨组织病理学评分、BMD、TMD、BV/TV、Tb.N、Tb.Th、Wnt3a和β-catenin mRNA与蛋白表达水平降低,Tb.Sp、TNF-α、IL-1β和IL-6水平升高(P<0.05)。结论针刺能够改善PMOP股骨骨折大鼠骨密度,减轻体内炎症反应,提高成骨影响因子水平,可能是通过调节Wnt信号通路发挥作用。Objective To explore the therapeutic effect of acupuncture on bone injury in postmenopausal osteoporosis fracture rats and its possible mechanism.Methods Thirsty-six female SD rats were selected to establish the postmenopausal osteoporosis model by bilateral ovariectomy.Three weeks after the model was successfully established,the femoral fracture model was established by closed fracture method.They were randomly divided into model group,acupuncture group,acupuncture+Wnt antagonist group,and another sham operation group.Rats in the acupuncture group was treated with acupuncture.Rats in the acupuncture+Wnt antagonist group was treated with acupuncture while subcutaneously injecting 20 mg kg-1 Wnt antagonist DKK1.Rats in the sham operation group and the model group were only given routine feeding.Bone mineral density(BMD),tissue mineral density(TMD)and bone volume fraction(BV/TV),the number of trabecular bone(TB.N),trabecular bone thickness(TB.TH),and trabecular bone separation(TB.SP)of femurs in each group were measured.The levels of serum inflammatory factors and osteogenic influencing factors were detected in each group.HE staining was used to observe the histopathological changes of femurs and to evaluate the bone injury.RT-PCR and Western blotting were used to detect the secreted protein Wnt3a andβ-catenin mRNA and protein expressions.Results Compared with those in the sham operation group,ALP,PINP,histopathological score of the femur,BMD,TMD,BV/TV,TB.N,TB.th,and Wnt3a andβ-catenin mRNA and protein expressions decreased,the levels of TB.Sp,TNF-α,IL-1β,and IL-6 increased in the model group(P<0.05).Compared with those in the model group,ALP,PINP,histopathological score of the femur,BMD,TMD,BV/TV,TB.N,TB.th,and Wnt3a andβ-catenin mRNA and protein expressions increased,the levels of TB.Sp,TNF-α,IL-1β,and IL-6 decreased in the acupuncture group(P<0.05).Compared with those in the acupuncture group,ALP,PINP,histopathological score of the femur,BMD,TMD,BV/TV,TB.N,TB.th,and Wnt3a andβ-catenin mRNA and protein ex

关 键 词：针刺 绝经后骨质疏松症 股骨骨折 WNT信号通路 Β连环蛋白 

分 类 号：R683[医药卫生—骨科学]