富血小板血浆与贫血小板血浆治疗中华眼镜蛇细胞毒素致小鼠皮肤溃疡的效果比较  

Comparison of therapeutic effects of platelet-rich plasma or platelet-poor plasma on skin ulcers caused by Naja naja atra cytotoxin in mice

作　　者：张志文 黄君庭 杨磊[1] 吴伯威 周红甜 曹赞锋 杨展正[1] 陈少英[1] 蒋敏[1] 梁子敬[1] 梁庆[1] ZHANG Zhi-wen;HUANG Jun-ting;YANG Lei;WU Bo-wei;ZHOU Hong-tian;CAO Zan-feng;YANG Zhan-zheng;CHEN Shao-ying;JIANG Min;LIANG Zi-jing;LIANG Qing(Department of Emergency Medicine,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou City,Guangdong Province,510120,China)

机构地区：[1]广州医科大学附属第一医院急诊科,广东广州510120

出　　处：《蛇志》2023年第1期4-8,共5页Journal of Snake

基　　金：广东省基础与应用基础研究基金资助面上项目(项目名称:中华眼镜蛇蛇毒CTXs毒素体外筛选和毒性抑制靶点研究,项目编号:2022A1515012095);广州市科技计划项目资助市校(院)联合项目(项目名称:抗蛇毒血清和糖皮质激素对蛇伤溃疡相关蛇毒和肌毒素的体外保护效能研究,项目编号:202102010282;项目名称:中国蛇伤救治智能化全数据库,项目编号:202102010001)。

摘　　要：目的 比较富血小板血浆(PRP)与贫血小板血浆(PPP)治疗中华眼镜蛇细胞毒素致小鼠皮肤溃疡的效果。方法 将中华眼镜蛇细胞毒素造模成功的30只中小鼠随机分为对照组(n=10)、PRP组(n=10)、PPP组(n=10),造模后第9天各组分别给予单纯生理盐水、PRP、PPP治疗,比较3组小鼠皮肤溃疡面积、溃疡创面愈合率、创面面积差绝对值与HE染色的病理改变。结果 低速组的PRP、PPP符合工作浓度标准。PRP凝胶、PPP凝胶治疗小鼠溃疡愈合时间可缩短3 d。PRP组与PPP组创面愈合率及3 d内面积差绝对值均大于对照组(均P<0.05)。镜下见PRP凝胶、PPP凝胶可减少炎症细胞浸润,促进小鼠上皮细胞再上皮化等病理改变。结论 PRP凝胶、PPP凝胶均可促进中华眼镜蛇伤溃疡皮肤修复,缩短愈合时间。Objective To compare the efficacy of platelet-rich plasma(PRP)and platelet-poor plasma(PPP)in the treatment of skin ulcers caused by Naja naja atra cytotoxin in mice.Methods 30 Naja naja atra cytotoxin modeling mice were randomly divided into control group(n=10),PRP group(n=10)and PPP group(n=10).On the 9th day after modeling,each group was applied with simple saline,PRP and PPP,respectively.The healing rate,area difference,skin ulcer gross and HE staining pathological changes were compared in each group.Results The PRP and PPP of the low-speed group met the working concentration standards.The healing time of PRP gel and PPP gel in mice can be shortened by 3 days.The absolute values of the 3-day area difference between the PRP group and the PPP group was respectively larger than that of Control group(P<0.05 for both).Under microscope,PRP gel and PPP gel can reduce the infiltration of inflammatory cells and promote the re-epithelialization of mouse epithelial cells.Conclusion PRP gel and PPP gel both can promote skin repair of snake wound ulcer caused by Naja naja atra cytotoxin envenomation and reduce healing time.

关 键 词：中华眼镜蛇 细胞毒素 皮肤溃疡 富血小板血浆 贫血小板血浆 

分 类 号：R996.3[医药卫生—毒理学]