Possible mechanisms associated with immune escape and apoptosis on anti-hepatocellular carcinoma effect of Mu Ji Fang granules  

作　　者：Yi-Bing Zhang Yong-Rui Bao Shuai Wang Tian-Jiao Li He Tai Jia-Peng Leng Xin-Xin Yang Bo-Cai Wang Xian-Sheng Meng 

机构地区：[1]College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,Liaoning Province,China [2]Department of Clinical Trail Institution Office,Dalian Municipal Central Hospital,Dalian 116033,Liaoning Province,China [3]Technical Innovation Center of Multidimensional Analysis of Traditional Chinese Medicine of Liaoning Province,Dalian 116600,Liaoning Province,China [4]Engineering Laboratory of Modern Chinese Medicine Research of Liaoning Province,Dalian 116600,Liaoning Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2023年第3期504-522,共19页世界胃肠肿瘤学杂志（英文版）（电子版）

基　　金：Supported by National Natural Science Foundation of China,No.81874342;Natural Science Foundation of Liaoning Province,No.2020-MZLH-35.

摘　　要：BACKGROUND Hepatocellular carcinoma(HCC)is one of the most common digestive system cancers with high mortality rates worldwide.The main ingredients in Mu Ji Fang Granules(MJF)are alkaloids,flavonoids,and polysaccharides.MJF has been used in the clinical treatment of hepatitis,cirrhosis and HCC for more than 30 years.Few previous studies have focused on the mechanism of MJF on tumor immunology in the treatment of HCC.AIM To explore the mechanism of action of MJF on tumor immunology in the treatment of HCC.METHODS The absorbable ingredients of MJF were identified using Molecule Network related to High Performance Liquid Chromatography-Electron Spray Ionization-Time of Flight-Mass Spectrometry,and hub potential anti-HCC targets were screened using network pharmacology and pathway enrichment analysis.Forty male mice were randomly divided into the Blank,Model,and MJF groups(1.8,5.4,and 10.8 g/kg/d)following 7 d of oral administration.Average body weight gain,spleen and thymus indices were calculated,tumor tissues were stained with hematoxylin and eosin,and Interferon gamma(IFN-γ),Tumor necrosis factorα(TNF-α),Interleukin-2,aspartate aminotransferase,alanine aminotransferase,alpha-fetoprotein(AFP),Fas,and FasL were measured by Enzyme-linked Immunosorbent Assay.Relevant mRNA expression of Bax and Bcl2 was evaluated by Real Time Quantitative PCR(RTqPCR)and protein expression of Transforming growth factorβ1(TGF-β1)and Mothers against decapentaplegic homolog(SMAD)4 was assessed by Western blotting.The HepG2 cell line was treated with 10 mg/mL,20 mg/mL,30 mg/mL,40 mg/mL of MJF,and another 3 groups were treated with TGF-β1 inhibitor(LY364947)and different doses of MJF.Relevant mRNA expression of TNF-α,IFN-γ,Bax and Bcl2 was evaluated by RT-qPCR and protein expression of TGF-β1,SMAD2,p-SMAD2,SMAD4,and SMAD7 was assessed by Western blotting.RESULTS It was shown that MJF improved body weight gain and tumor inhibition rate in H22 tumorbearing mice,protected immune organs and liver function,reduced the HCC indicator AFP,

关 键 词：Mu Ji Fang granules Hepatocellular carcinoma Transforming growth factorβ1/Mothers against decapentaplegic homolog Immune escape H22 tumor-bearing mice HepG2 cells 

分 类 号：R285.5[医药卫生—中药学]