基于四象限液晶器件的差分相衬成像系统  

Differential phase contrast imaging system based four quadrants liquid crystal device

作　　者：吴沛霖 彭增辉[1] 穆全全[1] 宣丽[1] 张世冬 李大禹[1] WU Pei-lin;PENG Zeng-hui;MU Quan-quan;XUAN Li;ZHANG Shi-dong;LI Da-yu(Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China;University of Chinese Academy of Sciences,Beijing 100049,China;Zhongke Juyan Stem Cell Technology Co.,Ltd.,Jilin 132000,China)

机构地区：[1]中国科学院长春光学精密机械与物理研究所,吉林长春130033 [2]中国科学院大学,北京100049 [3]中科聚研(吉林)干细胞科技有限公司,吉林吉林132000

出　　处：《液晶与显示》2023年第4期456-461,共6页Chinese Journal of Liquid Crystals and Displays

基　　金：国家重点研发计划(No.2021YFB3600300);国家自然科学基金(No.11974345,No.61975202,No.U2241224);吉林省与中国科学院科技合作高新技术产业化专项(No.2023SYHZ0039)。

摘　　要：在显微成像领域中,高成像质量图像的获取与良好的照明方式息息相关。传统显微镜使用聚光透镜来提供均匀强度的照明,调节聚光透镜的光阑匹配不同放大倍率的物镜。然而无色生物细胞的光学吸收系数低,在传统显微镜下难以观测到其细节信息。为了突破传统显微镜的成像功能,本文设计了一种可调控的显微镜聚光镜模块,通过将小型扭曲液晶器件嵌入聚光透镜的后焦面处,调控液晶器件的对光的透过效果可以实现明场成像以及差分相衬成像。系统由一款商用显微镜改装而成,液晶器件尺寸为22 mm×18 mm,实现了系统的高度集成化。通过实验验证了系统的成像性能,实现了对微凸透镜样品的定量相位重建,实验与理论曲线的互相关系数达到0.9949,并且通过胚胎干细胞的重建展示了系统在实际应用中的效果。In the field of microscopic imaging,the acquisition of high-quality images is closely related to good illumination mode.Traditional microscopes use a condensing lens to provide illumination with uniform intensity and adjust the aperture of the condensing lens to match the objective lenses with different magnification.However,it is difficult to observe the details of colorless biological cells which optical absorption coefficient is low under the traditional microscope.In order to improve the imaging capacity of traditional microscopes,this paper designs a kind of adjustable microscope condenser module.Bright field imaging and differential phase contrast imaging can be achieved by embedding a small twisted liquid crystal device in the back focal plane of the condenser lens to regulate the light transmission effect of the liquid crystal device.The system is refitted from a commercial microscope.The size of the liquid crystal device is 22 mm×18 mm,which realizes the high integration of the system.The imaging performance of the system is verified through experiments and the correlation coefficient between experimental and theoretical curves reaches 0.9949.The reconstruction of embryonic stem cells shows the effect of the system in practical application.

关 键 词：显微镜 计算成像 差分相衬成像 

分 类 号：O438[机械工程—光学工程]