二化螟对双酰胺类杀虫剂抗性的分子机制研究进展  

Research progress in molecular mechanisms of resistance to diamide insecticides in Chilo suppressalis

作　　者：徐鹿[1] 孙杨[3] 刘宝生[1] 徐德进[1] 徐广春[1] 赵钧 张亚楠[5] 张月亮[1] 黄水金[3] 吴顺凡 高聪芬[2] XU Lu;SUN Yang;LIU Baosheng;XU Dejin;XU Guangchun;ZHAO Jun;ZHANG Yanan;ZHANG Yueliang;HUANG Shuijin;WU Shunfan;GAO Congfen(Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base,Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;Institute of Plant Protection,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;Institute of Tobacco Research,Henan Academy of Agricultural Sciences,Xuchang 461000,Henan Province,China;College of Life Sciences,Huaibei Normal University,Huaibei 235000,Anhui Province,China)

机构地区：[1]江苏省农业科学院植物保护研究所,省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室,南京210014 [2]南京农业大学植物保护学院,南京210095 [3]江西省农业科学院植物保护研究所,南昌330200 [4]河南省农业科学院烟草研究所,河南许昌461000 [5]淮北师范大学生命科学学院,安徽淮北235000

出　　处：《农药学学报》2023年第2期257-266,共10页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金面上项目(31972309,31972308,31860501);江苏省农业科学院基本科研业务专项资金项目(ZX(22)4132);安徽省教育厅高校自然科学研究项目(KJ2021ZD0059).

摘　　要：新型双酰胺类杀虫剂已广泛用于保障水稻生产,而二化螟作为危害水稻生产的钻蛀性害虫,已经对该类杀虫剂产生了抗性,明确该类杀虫剂抗性分子机制,可为二化螟抗性快速检测和绿色防控提供技术支撑。本文在总结二化螟对双酰胺类杀虫剂抗性现状的基础上,重点综述了近年来有关其抗性分子机制研究的进展,主要包括解毒酶和转运蛋白基因过表达介导的代谢抗性,以及鱼尼丁受体基因突变介导的靶标抗性;指出了该研究领域在抗性分子检测、抗性新基因鉴定、抗性基因调控网络和多重抗性机制等方面存在的问题,并展望了其发展方向,认为:利用高通量测序技术检测害虫种群抗药性;利用多组学技术鉴定新抗性基因及调控网络,以探明多重抗性机制;将反向遗传学工具放射性配基结合及电生理技术深入验证抗性基因功能;需开发靶向抗性基因的dsRNA转基因作物、纳米农药及选择性新型化学杀虫剂,以达到杀虫剂减施增效的目的。New diamide insecticides have been widely used to safeguard rice production.Chilo suppressalis(Walker),as a rice striped stem borer that harms rice production,has developed resistance to this type of insecticide.Further study on the molecular mechanism of resistance can provide technical support for the rapid detection of resistance and green control of C.suppressalis.Based on the current situation in resistance of C.suppressalis to diamide insecticides,the latest research advances in the resistance molecular mechanisms are summarized,including the overexpression of detoxification enzyme and transport genes mediated metabolic resistance and mutations of the ryanodine receptor gene mediated target resistance.The current existing issues in this research area mainly focus on the molecular detection of resistance,the identification of new resistance genes,the function and regulatory network of resistance genes,and the mechanism of multiple resistance.Future development directions have been prospected including resistance detection in the pest population by high throughput sequencing technology,identification of new resistance genes and resistance regulation networks for conformation of multiple resistance mechanisms using multi-omics technology,further functional verification of resistance genes by reverse genetics tools combined with radioligand binding analysis and electrophysiological technique,dsRNA transgenic crops targeting resistance genes,nano insecticides,and selective new chemical insecticides to reduce insecticides application and improve the efficiency of insecticides.

关 键 词：二化螟 双酰胺类杀虫剂 代谢抗性 靶标抗性 抗性分子机制 

分 类 号：S481.4[农业科学—农药学] Q965.9[农业科学—植物保护]