樱桃黑斑病菌对氟唑菌酰羟胺的敏感性  

Sensitivity of Alternaria alternata causing black spot disease on cherry to pydiflumetofen

作　　者：轷思玲 李阿根[2] 杨晓琦 张传清[1] HU Siling;LI Agen;YANG Xiaoqi;ZHANG Chuanqing(College of Advanced Agricultural Sciences,Zhejiang Agricultural and Forestry University,Hangzhou 311300,China;Yuhang’s Management Station for the Agricultural Ecology and Plant Protection of Hangzhou,Hangzhou 311100,China)

机构地区：[1]浙江农林大学现代农学院,杭州311300 [2]杭州市余杭区农业生态与植物保护管理总站,杭州311100

出　　处：《农药学学报》2023年第2期364-369,共6页Chinese Journal of Pesticide Science

摘　　要：采用菌丝生长速率法测定了樱桃黑斑病菌交链格孢Alternaria alternata群体(n=103)对新型琥珀酸脱氢酶抑制剂(SDHI)氟唑菌酰羟胺的敏感性,评价了该药剂对A.alternata菌株孢子萌发的抑制活性及其对樱桃黑斑病的保护和治疗作用。结果表明:氟唑菌酰羟胺对103株A.alternata菌株群体的EC_(50)值在0.027~1.175μg/mL之间,平均EC_(50)值为(0.236±0.101)μg/mL,敏感性频率分布呈现单峰曲线,该平均EC_(50)值可作为樱桃A.alternata菌株对氟唑菌酰羟胺的敏感性基线;进一步分析发现,琥珀酸脱氢酶中的铁硫蛋白(SDHB)和两个嵌膜蛋白(SDHC和SDHD)亚基未发现与敏感性差异有关的氨基酸突变。氟唑菌酰羟胺对樱桃A.alternata的孢子萌发也有较强的抑制活性,并且对樱桃黑斑病的保护作用防治效果强于治疗作用防治效果。这些结果可为氟唑菌酰羟胺在樱桃黑斑病及其他病害管理上的推广和科学用药提供依据。In this study,the sensitivity of Alternaria alternata population(n=103)to pydiflumetofen,a novel succinate dehydrogenase inhibitor(SDHI),was determined by the mycelial growth rate method.The inhibitory activity of this fungicide on spore germination and its protective and curative effects on black spot were evaluated.The results showed that the EC_(50) value of pydiflumetofen against A.alternata population(n=103)was between 0.027μg/mL and 1.175μg/mL,and the average EC_(50) value was(0.236±0.101)μg/mL.The sensitivity frequency distribution showed a unimodal curve,and the average EC_(50) value could be used as the sensitivity baseline of A.alternata to pydiflumetofen.Further analysis showed that no amino acid mutation was detected in succinate dehydrogenase subunit B/C/D(SDHB,SDHC,SDHD)subunits of the strains with sensitivity differences.Pydiflumetofen also had strong inhibitory activity on spore germination and its protective effect was superior to the curative effect.These results provide a basis for the promotion and scientific application of pydiflumetofen in the management of cherry black spot and other diseases.

关 键 词：樱桃黑斑病 交链格孢 氟唑菌酰羟胺 敏感性基线 SDH基因突变 防治效果 

分 类 号：S481.4[农业科学—农药学]