杨树农杆菌遗传转化研究30年  

Thirty Years of Agrobacterium-Mediated Genetic Transformation of Populus

作　　者：陈赢男[1] 胡传景 诸葛强[1] 胡建军[2] 尹佟明[1] Chen Yingnan;Hu Chuanjing;Zhuge Qiang;Hu Jianjun;Yin Tongming(Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education Co-Innovation Center for the Sustainable Forestry in Southern China Nanjing Forestry University,Nanjing 210037;Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091)

机构地区：[1]南京林业大学、林木遗传与生物技术省部共建教育部重点实验室、南方现代林业协同创新中心,南京210037 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学》2022年第12期114-129,共16页Scientia Silvae Sinicae

基　　金：十四五国家重点研发计划项目(2021YFD2200202);国家自然科学基金项目(32071795)。

摘　　要：杨树是我国最为重要的国土绿化和工业用材树种之一,精准高效培育速生、优质和高抗的杨树新品系是今后杨树育种理论和技术发展的重要方向。以农杆菌介导的遗传转化体系为基础的基因工程育种已成为缩短林木育种周期、提升育种效率的核心技术。自20世纪80年代证实杨树是农杆菌的天然寄主以来,国内外开展了大量构建农杆菌介导杨树稳定遗传转化体系的研究。本文综合分析了已有研究报道,详细回顾了不同派系杨树树种遗传转化体系的构建历程,系统整理了影响杨树遗传转化体系的主要因素,并对杨树基因工程育种的研究前景进行了展望,以期为进一步提升杨树遗传转化效率提供信息依据,为开发顽拗性木本植物和生产推广良种的遗传转化体系提供参考方案。Poplar(Populus spp.)is one of the most important tree species used for land greening and industrial timber production in China,and efficiently and precisely breeding new cultivars with rapid growth,high quality and strong stress tolerance will be an important direction for the future theoretical and technological development in poplar breeding.The Agrobacterium-mediated genetic transformation system has been the critical technology for shortening breeding cycle and increasing breeding efficiency.A large number of researches have been conducted on developing the Agrobacterium-mediated genetic stable transformation system for poplar since it was proved to be a native host of Agrobacterium in 1980s.We performed a comprehensive analysis of existing literatures,reviewed the historical process of constructing Agrobacterium-mediated genetic transformation system in different poplar species,summarized the main factors affecting transformation efficiency,and also discussed some future perspectives in this area.This review will provide useful information for further improving the poplar transformation efficiency and present reference protocols for establishing transformation systems in recalcitrant plant species and elite cultivars widely applied in production.

关 键 词：杨树 遗传转化 根癌农杆菌 发根农杆菌 组培再生 

分 类 号：S722.3[农业科学—林木遗传育种]