小麦光温敏雄性不育系BS237ω-黑麦碱基因克隆及序列分析  

Cloning and Sequence Analysis ofω-Secalin Gene in Wheat Photothermal-Sensitive Male Sterile Line BS237

作　　者：侯起岭 杨卫兵 娄红耀 杜冰 高建刚[1] 赵昌平[1] 张风廷[1] 秦志列[1] Hou Qiling;Yang Weibing;Lou Hongyao;Du Bing;Gao Jiangang;Zhao Changping;Zhang Fengting;Qin Zhilie(Institute of Hybrid Wheat,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)

机构地区：[1]北京市农林科学院杂交小麦研究所,北京100097

出　　处：《山东农业科学》2023年第3期1-8,共8页Shandong Agricultural Sciences

基　　金：北京市农林科学院青年基金项目(QNJJ202225);北京市农林科学院科技创新能力建设专项(KJCX20200418)。

摘　　要：为研究小麦光温敏雄性不育系BS237中ω-黑麦碱基因编码区特征,利用同源克隆的方法克隆ω-黑麦碱基因的编码区序列,共得到8条具有完整开放阅读框的基因序列(OK999982~OK999985,OK999987~OK999990),基因编码区长度为1002~1074 bp,可编码333~357个氨基酸残基,全部编码RQL型ω-黑麦碱蛋白。NCBI BLAST分析表明克隆序列与已知序列的相似度在91%~100%之间,推断其为ω-黑麦碱基因家族。进化分析表明8个克隆的基因与普通小麦(Triticum aestivum L.)和黑麦(Secale cereale L.)的ω-黑麦碱基因序列具有较高的同源性,说明ω-黑麦碱基因具有一定的基因组特异性。乳糜泻(CD)免疫肽识别分析表明,8个基因中均分布有5种T细胞免疫肽——Gli-ωt(PQQPFPQQ)、DQ2.5-glia-γ5(QQPFPQQPQ)、QQPY、SPQQ和PQQP,且肽段Gli-ωt(PQQPFPQQ)和PQQP存在多个拷贝。本研究结果可为1BL/1RS类型杂交小麦加工品质的分子改良及乳糜泻疾病的预防提供参考。In order to study the characteristics ofω-secalin gene coding region in wheat photothermal-sensitive male sterile line BS237,the sequence ofω-secalin gene coding region was cloned by homologous clo-ning method.The results showed that eight different gene sequences(OK999982~OK999985,OK999987~OK999990)were cloned,which had complete open reading frames,and the length variation of gene coding region ranged from 1002 bp to 1074 bp,encoding 333~357 amino acid residues and all encoding RQLω-secalin protein.NCBI BLAST analysis showed that the similarity between the cloned sequence and known se-quence ranged from 91%to 100%,suggesting that the cloned sequences beω-secalin gene family.Cluster a-nalysis showed that the eight cloned genes had high homology withω-secalin gene sequences of Triticum aesti-vum L.and Secale cereale L.,suggesting thatω-secalin genes have certain genomic specificity.The celiac disease(CD)toxicity analysis showed that five immunopeptide were distributed in all the eight genes,including Gli-ωt(PQQPFPQQ),DQ2.5-glia-γ5(QQPFPQQPQ),QQPY,SPQQ and PQQP.Multiple copies of Gli-ωt(PQQPFPQQ)and PQQP existed in all the eight genes.The results of this study could provide references for molecular improvement of processing quality in 1BL/1RS type hybrid wheat and prevention of celiac disease.

关 键 词：小麦 光温敏雄性不育系 ω-黑麦碱 基因克隆 序列分析 乳糜泻 

分 类 号：S512.1[农业科学—作物学] Q785[生物学—分子生物学]