基于CD40/CD40L探讨重楼总皂苷对TNBS诱导的大鼠急性实验性结肠炎作用机制  

Approach to effect and mechanism of rhizoma paridis total saponins on TNBS-induced acute experimental colitis in rats based on CD40/CD40L

作　　者：刘可心 王卯[2] 吴星星 吴琪 王耀辉[2] 刘丽波[1] 吴静[3] LIU Ke-xin;WANG Mao;WU Xing-xing(The 981st Hospital of the Joint Logistic Support Force of the Chinese People's Liberation Army,Chengde 067000,China;不详)

机构地区：[1]中国人民解放军联勤保障部队第九八一医院,承德067000 [2]南京中医药大学附属医院 [3]江苏省南京鼓楼医院

出　　处：《山西中医》2023年第4期51-56,共6页Shanxi Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(编号:81873160);2021年江苏省研究生科创计划项目(编号:KYCX21_1661)。

摘　　要：目的:探讨重楼总皂苷(rhizomaparidis total saponins,RPTS)对三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)诱导的急性实验性结肠炎大鼠炎症与凝血的影响及其作用机制。方法:将50只雄性SPF级SD大鼠,随机分为空白组7只,模型组13只,以及美沙拉嗪组、RPTS低剂量组及RPTS高剂量组,每组各10只。通过TNBS建立大鼠急性实验性结肠炎模型,共14天。实验评估终点生存率、DAI评分、CMDI评分、TDI评分等指标,探究RPTS对大鼠急性实验性结肠炎的量效关系。检测全血细胞及凝血等相关指标;采用ELISA方法检测血浆及肝脏组织中CD40/CD40L指标,采用RT-PCR方法检测结肠组织中IL-1β及TNF-α的m RNA水平。结果:模型组终点生存率76.9%,RPTS低剂量组生存率为100%;RPTS低剂量组DAI、CMDI及TDI评分均较模型组显著降低(P﹤0.05);在肝脏组织中的CD40/CD40L表达水平与模型组比较,美沙拉嗪组及RPTS低剂量组均显著增加(P﹤0.0001);同时,在结肠组织的IL-1βm RNA检测中,与模型组比较,RPTS低剂量组IL-1β mRNA降低(P﹤0.05)。结论:RPTS改善了TNBS诱导急性实验性结肠炎大鼠的症状,具有良好的治疗作用,提高了模型鼠的终点生存率;RPTS低剂量组治疗效果更佳,可能通过激活CD40/CD40L共刺激因子,调控实验性结肠炎大鼠的免疫机制,以缓解疾病的炎症反应。Objective:To approach the effect and mechanism of rhizoma paridis total saponins(RPTS)on inflammation and coagulation in rats with 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced acute experimental colitis.Methods:50 male SD rats of SPF grade were randomly divided into normal control group(n=7),TNBS model group(n=13),Mesalazine group(n=10),RPTS low-dose group(n=10)and RPTS high-dose group(n=10).The rat model of acute experimental colitis was established by TNBS for 14 days.The indexes such as survival rate of final point,DAI score,CMDI score,TDI score,etc.were evaluated.The dose-efficacy relationship that RPTS was related to acute experimental colitis of rats was approached.The relevant indexes such as whole blood cell and coagulation,etc.were detected.CD40/CD40L indexes in plasma and liver tissue were detected by ELISA.mRNA levels of IL-1βand TNF-αin colon tissue was detected by RT-PCR.Results:The survival rate of final point was 76.9%in TNBS model group and 100%in RPTS low-dose group.The scores of DAI,CMDI and TDI of RPTS low-dose group were significantly decreased compared with TNBS model group(P﹤0.05).The expression level of CD40/CD40L in liver tissue,compared with TNBS model group,this expression level of Mesalazine group and RPTS low-dose group was a good therapeutic effect,and improve the survival rate of final point of model rats;and the therapeutic effect of RPTS low-dose group is better.It may be that the immune mechanism of rats with experimental colitis is regulated and controlled by activating CD40/CD40L costimulatory factor,so as to alleviate the inflammatory reaction of the disease.

关 键 词：实验性结肠炎 重楼总皂苷 2 4 6-三硝基苯磺酸 CD40/CD40L 实验研究 

分 类 号：R285.5[医药卫生—中药学]