Species-specific regulatory pathways of small RNAs play sophisticated roles in flower development in Dimocarpus longan Lour.  

作　　者：Bo Liu Guanliang Li Chengjie Chen Zaohai Zeng Jing Xu Jisen Zhang Rui Xia Yuanlong Liu 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,South China Agricultural University,Guangzhou,Guangdong 510640,China [2]Guangdong Laboratory for Lingnan Modern Agriculture,South China Agricultural University,Guangzhou,Guangdong 510640,China [3]Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China,Ministry of Agriculture and Rural Affairs,South China Agricultural University,Guangzhou,Guangdong 510640,China [4]Center for Genomics and Biotechnology,Haixia Institute of Science and Technology,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China

出　　处：《Horticultural Plant Journal》2023年第2期237-249,共13页园艺学报（英文版）

基　　金：funded by the National Key Research and Developmental Program of China(Grant No.2018YFD1000104);supported by awards from the National Natural Science Foundation of China(Grant Nos.32002009 and 32072547);The Special Support Program of Guangdong Province(Grant No.2019TX05N193).

摘　　要：Flower development plays vital role in horticultural plants.Post-transcriptional regulation via small RNAs is important for plant flower development.To uncover post-transcriptional regulatory networks during the flower development in Dimocarpus longan Lour.‘Shixia’,an economically important fruit crop in subtropical regions,we collected and analyzed sRNA deep-sequencing datasets and degradome libraries Apart from identifying miRNAs and phased siRNA generating loci(PHAS loci),120 hairpin loci,producing abundant sRNAs,were identified by in-house protocols.Our results suggested that 56 miRNA-target pairs,2221-nt-PHAS loci,and 111 hairpin loci are involved in posttranscriptional gene silencing during longan reproductive development.Lineage-specific or species-specific post-transcriptional regulatory modules have been unveiled,including miR482-PHAS and miRN15.miR482-PHAS might be involved in longan flower development beyond their conserved roles in plant defense,and miRN15 is a novel miRNA likely associated with a hairpin locus(HPL-056)to regulate strigolactone receptor gene DWARF14(D14)and the biogenesis of phasiRNAs from D14.These small RNAs are enriched in flower buds,suggesting they are likely involved in post-transcriptional regulatory networks essential for longan flower development via the strigolactone signaling pathway.

关 键 词：Dimocarpus longan Lour Small RNAs Flower development Post-transcriptional regulation 

分 类 号：S667.2[农业科学—果树学]