Genome-wide characterization of graft-transmissible mRNA-coding P450 genes of cucumber(Cucumis sativus L.)  

作　　者：Tao Wang Xiaohong Lu Chenggang Xiang Xiaojun Li Wenqian Liu QingWang Zixi Liu Lihong Gao Wenna Zhang 

机构地区：[1]Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops,China Agricultural University,Beijing,100193 China [2]College of Life Science and Technology,HongHe University,Mengzi,Yunnan,661199 China

出　　处：《Horticultural Plant Journal》2023年第2期250-260,共11页园艺学报（英文版）

基　　金：supported by National Key Research and Development Program of China(Grant Nos.2018YFD1000800 and 2019YFD1000300);National Natural Science Foundation of China(Grant No.31872158);Earmarked Fund for China Agriculture Research System(Grant No.CAS-23).

摘　　要：P450(cytochrome P450)is a supergene family,which is involved in various metabolic pathways in plants.Based on previous study,we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts.However,the reason that why P450 mRNAs were endorsed as signaling,and what specific motif(s)did they harbored is not clear yet.Here,we first identified 221 CsaP450 genes in cucumber genomewide level.Combining with graft-transmissiblemRNAs datasets in cucumber,we elucidated 15mobile-CsaP450-coding genes,of which 5 and 10 belonged to A-type and non-A type respectively.Compared with Arabidopsis and pumpkin(Cucubit moschata)graft-induced-transmissible P450 mRNAs,a phylogenetic treewas constructed and divided into eight clans by usingmultiple-sequence alignment.Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)annotations indicated that the expression patterns of the mobile-mRNA-coding CsaP450 genes in different tissues of cucumber was specifically enriched in oxidoreductase activity and secondary metabolic pathways.The structures and motifs of these 15 mobile-mRNA-coding CsaP450 genes and their types of regulatory elements told that the proportion of CU-rich motifs was higher than nonmobile-mRNA-coding CsaP450 genes.The integrated analysis of mobility direction and mRNA abundance of 15 mobilemRNA-coding CsaP450 genes allowed to conclude that there was rarely relationship between them.The study provided a new insight into the relationship between the motifs and functional characterization of mobile-mRNA-coding P450 genes of cucumber in genome-wide levels.

关 键 词：Cucumis sativus GENOME-WIDE Cytochrome P450 mRNA mobility Phylogeny CU-rich motif 

分 类 号：S642.2[农业科学—蔬菜学]