Functional analysis of flavanone 3-hydroxylase(F3H)from Dendrobium officinale,which confers abiotic stress tolerance  

作　　者：Can Si Wei Dong Jaime A.Teixeira da Silva Chunmei He Zhenming Yu Mingze Zhang Lei Huang Conghui Zhao Danqi Zeng Chuanmao Li Shengxiang Qiu Jun Duan 

机构地区：[1]Key laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement&Guangdong Provincial,Key laboratory of Applied Botany,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]College of Life Science and Technology,Xi An Jiao Tong University,Xi’an,Shaanxi 710049,China [4]Independent Researcher,Miki-cho,Kagawa-ken,Japan [5]Guangzhou Keneng Cosmetic Scientific Research Co.,Ltd,Guanghzou,Guangdong 510650,China

出　　处：《Horticultural Plant Journal》2023年第2期356-364,共9页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.31871547)。

摘　　要：Flavonoids are important bioactive components in Dendrobium officinale,a medicinal orchid.They are involved in many biological activities,including protecting plants against biotic and abiotic stresses.Research on the key genes related to flavonoid biosynthesis in D.officinale is limited.In this study,one of the key flavonoid biosynthesis genes,flavanone 3-hydroxylase(F3H),was characterized from D.officinale.The open reading frame of DoF3H was 1134 bp long and it encoded a 377-amino acid protein.The DoF3H protein showed considerably high homology with F3H proteins from other plant species and shared a common evolutionary ancestor with other F3Hs.DoF3H transcripts were detected in different organs of adult plants and mainly accumulated in flowers,followed by roots,stems and leaves,a pattern that was similar to the content of flavonoids.Recombinant DoF3H protein,which was localized in the cytosol,could convert naringenin to dihydrokaempferol.The mRNA levels of DoF3H were significantly induced by salt and cold stresses.Furthermore,the heterologous expression of DoF3H in Escherichia coli conferred it higher tolerance to salt and cold stresses.These results provide insight into the molecular function of DoF3H in the biosynthesis of flavonoids,and provide a new application for improvement of abiotic tolerance in D.officinale.

关 键 词：Dendrobium officinale Flavanone 3-hydroxylase Dihydrokaempferol Salt stress Cold stress 

分 类 号：S567.239[农业科学—中草药栽培]