滇朴和旱冬瓜叶片挥发物对云南切梢小蠹卵黄原蛋白及其受体基因转录的影响  

Influence of Volatiles from Leaves of Celtis kunmingensis and Alnus nepalensis on Gene Expression of Vitellogenin and Its Receptor of Tomicus yunnanensis

作　　者：王玉琴 赵琰明 张新民[1] 赵宁[1] 朱家颖[1,2] 杨斌[1,2] Wang Yuqin;Zhao Yanming;Zhang Xinmin;Zhao Ning;Zhu Jiaying;Yang Bin(Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Southwest Forestry University,Kunming Yunnan 650233,China;Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Southwest Forestry University,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学云南省森林灾害预警与重点实验室,昆明650233 [2]西南林业大学西南山地资源保育与利用教育部重点实验室,昆明650233

出　　处：《西南林业大学学报（自然科学）》2023年第2期62-68,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31760210)资助;云南省高层次人才培养支持计划“青年拔尖人才”专项项目(YNWRQNBJ-2018-393)资助。

摘　　要：通过荧光定量PCR分别检测它们在不同发育阶段(卵、幼虫、蛹及成虫)以及雌成虫不同组织(头、胸、翅、卵巢、腹部残体)中的表达量,并检测了它们于两种非寄主叶片挥发物处理不同时间后的表达量,分析非寄主滇朴和旱冬瓜叶片挥发物对云南切梢小蠹卵黄原蛋白基因(Vg)及其受体基因(VgR)表达量的影响。结果表明:Vg和VgR基因在蛹和成虫中的表达量高于其他各发育阶段,且在成虫中的表达量高于蛹中的。Vg基因在腹部残体的表达量最高,而VgR基因在卵巢中特异性表达。滇朴叶片挥发物处理20 d和40 d后,Vg基因的表达量显著降低,但处理60 d后该基因的表达量显著升高。旱冬瓜叶片挥发物20 d和40 d后,VgR基因的表达量显著减低,但处理60 d后该基因的表达量与对照相比不存在显著差异。综上所述,非寄主挥发物能通过调控云南切梢小蠹Vg和VgR基因的表达进而影响其产卵。In order to reveal the influence of volatiles from leaves of the non-host plants including Celtis kunmingensis and Alnus nepalensis on gene expression of the vitellogenin(Vg)and its receptor(VgR)of Tomicus yunnanensis,the transcriptions of these 2 genes at different development stages(egg,larvae,pupae and adult)and in different tissues of female adults(head,thorax,wing,ovary and abdomen carcass),and in response to the leaf volatiles from 2 non-host plants were determined using fluorescence quantitative real-time PCR.The results showed that the expressions of Vg and VgR genes are significant higher in pupae and adult than that in other developmental stages,and they were expressed significantly higher in adult than in pupae.Vg gene was expressed highest in abdomen carcass,while VgR gene has abundantly ovary-specific expression.The expression of Vg gene significantly reduced by the treatment of leaf volatiles from C.kunmingensis after 20 d and 40 d,while its expression significantly upregulated after 60 d.After the treatment of leaf volatiles from A.nepalensis 20 d and 40 d,the gene expression of VgR was significantly downregulated,while the expression of this gene didn't change after 60 d.The findings imply that egg production of T.yunnanensis can be influenced by non-host volatiles via regulation of its Vg and VgR gene expression,which lay a foundation for further revealing the underlying mechanisms.

关 键 词：云南切梢小蠹 非寄主挥发物 卵黄原蛋白 卵黄原蛋白受体 基因表达 

分 类 号：S763.3[农业科学—森林保护学]