玉米品种真实性SSR分子检测体系优化  

Optimization of SSR Molecular Detection System for Maize Variety Authenticity

作　　者：林显凤 罗友明 尚玥 赵长坤 龚辉 杨腾 白体坤 毛若涵 李仕伟 游宇 LIN Xian-feng;LUO You-ming;SHANG Yue(Nanchong Academy of Agricultural Sciences,Nanchong,Sichuan 637000;Seed Quality Supervision and Inspection Station of Nanchong,Nanchong,Sichuan 637000;Seed Administrative Station of Nanchong,Nanchong,Sichuan 637000)

机构地区：[1]南充市农业科学院,四川南充637000 [2]南充市种子质量监督检验站,四川南充637000 [3]南充市种子管理站,四川南充637000 [4]中国农业大学农学院,北京100163

出　　处：《安徽农业科学》2023年第6期93-99,共7页Journal of Anhui Agricultural Sciences

基　　金：南充市科技计划项目(21YFZJ0019)。

摘　　要：对南充市农业科学院提供的2份玉米品种,随机选用GB/T 39914-2021《主要农作物品种真实性和纯度SSR分子标记检测玉米》中的12个SSR位点,利用琼脂糖凝胶电泳检测法、变性PAGE银染检测法和毛细管电泳分析法,分析了不同的扩增程序、仪器设备和反应体系等对PCR产物质量的影响。结果表明:60℃退火温度和novoprotein Taq DNA聚合酶有利于玉米品种真实性SSR标记的检测;2种PCR扩增仪对扩增结果并无较大区别。60℃-PCR1-E1,即60℃退火温度,PCR扩增仪1[TP-96A(F)],novoprotein Taq DNA聚合酶,毛细管电泳法相结合为最优组合,可作为四川品种真实性快速检测候选方法。The 2 maize varieties provided by Nanchong Academy of Agricultural Sciences.12 SSR loci were selected from GB/T 39914-2021Variety Genuineness and Purity Testing of Main Crops with SSR Markers-Maize.Different methods including agarose gel electrophoresis,denaturing PAGE and capillary electrophoresis were used to analyze the effects of instruments,equipment and reaction system on the quality of PCR products.60℃annealing temperature and Novoprotein Taq DNA polymerase were more conducive to the detection of SSR markers for maize variety authenticity;There was no significant difference between the two PCR amplification instruments.60℃-PCR1-E1,i.e.60℃annealing temperature,novoprotein Taq DNA polymerase and PCR1 amplification instrument[TP-96A(F)]were the best combination,which could be used as candidate methods for variety authenticity detection in Northeast Sichuan.

关 键 词：SSR标记 玉米 真实性 检测体系 

分 类 号：S513[农业科学—作物学]