HPLC法同时测定人参败毒散标准汤剂中6种成分  

Simultaneous determination of six constituents in the standard decoction of Renshen Baidu Powder by HPLC

作　　者：张利恒 陈新[1] 邱智东[1] ZHANG Li-heng;CHEN Xin;QIU Zhi-dong(Key Laboratory for Pharmacological Substances of Genuine Herbs in Changbai Mountain,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学长白山道地药材药效物质重点研究室,吉林长春130117

出　　处：《中成药》2023年第4期1080-1085,共6页Chinese Traditional Patent Medicine

基　　金：重大疫情防治中药方剂储备库建设项目(吉中医药发[2021]11号)。

摘　　要：目的建立HPLC法同时测定人参败毒散标准汤剂中蛇床子素、二氢欧山芹醇当归酸酯、新橙皮苷、柚皮苷、甘草苷、阿魏酸的含量。方法该药物提取液的分析采用Agilent ZORBAX Extend-C_(18)色谱柱(4.6 mm×150 mm,5.0μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长273 nm。再进行聚类分析、主成分分析。结果6种成分在各自范围内线性关系良好(r≥0.9995),平均加样回收率97.00%~106.62%,RSD 0.26%~1.93%。10批样品聚为2类,主成分综合得分为0.709~0.789,RSD<3.7%。结论该方法简便准确,可为人参败毒散标准汤剂及其相关制剂的质量评价提供参考。AIM To establish an HPLC method for the simultaneous content determination of osthole,columbianadin,neohesperidin,naringin,liquiritin and ferulic acid in the standard decoction of Renshen Baidu Powder.METHODS The analysis of extract of this drug was performed on a 30℃ thermostatic Agilent ZORBAX Extend-C_(18) column(4.6 mm×150 mm,5.0μm),with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 273 nm.Then cluster analysis and principal component analysis were performed.RESULTS Six constituents showed good linear relationships within their own ranges(r≥0.9995),whose average recoveries were 97.00%-106.62% with the RSDs of 0.26%-1.93%.Ten batches of samples were clustered into two types,the comprehensive scores of principal components were 0.709-0.789 with the RSDs of less than 3.7%.CONCLUSION This simple and accurate method can provide a reference for the quality evaluation of the standard decoction of Renshen Baidu Powder and its related preparations.

关 键 词：人参败毒散 标准汤剂 化学成分 HPLC 聚类分析 主成分分析 

分 类 号：R927.2[医药卫生—药学]