淫羊藿苷对东莨菪碱致小鼠认知障碍及海马神经元兴奋性的作用  

Effects of icariin on scopolamine-induced cognitive impairment and neuronal excitability in mice

作　　者：王瑞雯 白亚楠 史海龙 侯文涛 李洋 WANG Rui-wen;BAI Ya-nan;SHI Hai-long;HOU Wen-tao;LI Yang(School of Pharmacy,North China University of Science and Technology,Tangshan 063210,China;Hebei Key Laboratory of Bioelectromagnetics and Neural Engineering,Hebei University of Technology,Tianjin 300130,China)

机构地区：[1]华北理工大学药学院,河北唐山063210 [2]河北工业大学河北省生物电磁与神经工程重点实验室,天津300130

出　　处：《中成药》2023年第4期1114-1119,共6页Chinese Traditional Patent Medicine

基　　金：河北省自然科学基金项目(H2021209059);华北理工大学博士科研启动基金项目(25759799)。

摘　　要：目的研究淫羊藿苷对东莨菪碱致小鼠认知障碍及对海马神经元兴奋性的作用。方法小鼠随机分为空白组、模型组、多奈哌齐组(2 mg/kg)及淫羊藿苷低、中、高剂量组(30、60、120 mg/kg),每组10只,连续给药14 d,第10天起,除空白组腹腔注射生理盐水外,其余各组均注射2 mg/kg东莨菪碱以建立认知障碍模型。采用Morris水迷宫实验和避暗实验评价小鼠的学习记忆能力,HE染色观察海马神经元形态变化,全细胞脑片膜片钳技术记录海马神经元静息膜电位和动作电位。结果与模型组比较,淫羊藿苷组小鼠在水迷宫实验中的逃避潜伏期降低(P<0.01),穿越平台次数增加(P<0.01);在避暗实验中的停留潜伏期增加(P<0.01),错误次数减少(P<0.01);小鼠海马DG区神经元损伤明显恢复,神经元数量增加(P<0.01),海马神经元电特性指标的衰退得到缓解(P<0.01)。结论淫羊藿苷能有效改善东莨菪碱致小鼠认知障碍,提高海马神经元兴奋性。AIM To investigate the effects of icariin on scopolamine-induced cognitive impairment and neuronal excitability in mice.METHODS The mice were randomly divided into the control group,the model group,the donepezil group(2 mg/kg),and the low,medium and high dose icariin groups(30,60,120 mg/kg),with 10 mice in each group.Each group was administered with the corresponding dose for 14 days consecutively,followed by the intraperitoneal use of physiological saline in the control group,and scopolamine injection in the other groups(2 mg/kg)for the establishment of cognitive impairment models starting on the 10th day.The mice had their learning and memory ability assessed by the Morris water maze task and dark avoidance experiment;their morphological changes of hippocampal neurons observed by HE staining;their resting membrane potential and action potential of the hippocampal neurons recorded by the whole cell patch-clamp technology.RESULTS Compared with the model group,the icariin groups displayed decreased escape latency(P<0.01);increased times of crossing the platform in the water maze experiment(P<0.01);increased stay latency in the dark avoidance experiment(P<0.01);decreased error times(P<0.01);obviously recovered neurons damage in DG area of hippocampus and therefore increased number of neurons(P<0.01);and alleviated decline of electrical characteristics of hippocampal neurons(P<0.01)as well.CONCLUSION Icariin can ameliorate scopolamine-induced cognitive impairment in mice and increase their neuronal excitability.

关 键 词：淫羊藿苷 认知障碍 神经元兴奋性 静息膜电位 动作电位 

分 类 号：R285.5[医药卫生—中药学]