GII.8和GII.9型诺如病毒P蛋白糖结合特征研究  

Glycan binding specificity of P protein in GII.8 and GII.9 noroviruses

作　　者：吴铮 苏韫曦 丛鑫 魏宇航 孔翔羽[2] 靳淼[2] 李丹地[2] 李金松[2] 孙晓曼[2] 段招军[2] Wu Zheng;Su Yunxi;Cong Xin;Wei Yuhang;Kong Xiangyu;Jin Miao;Li Dandi;Li Jinsong;Sun Xiaoman;Duan Zhaojun(School of Public Health,Gansu University of Chinese Medicine,Lanzhou 730020,China;National Health Commission Key Laboratory of Medical Viruses and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Diseases Control and Prevention,Beijing 102206,China)

机构地区：[1]甘肃中医药大学公共卫生学院,兰州730020 [2]中国疾病预防控制中心病毒病预防控制所、国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206

出　　处：《国际病毒学杂志》2023年第1期5-9,共5页International Journal of Virology

基　　金：国家重点研发计划(2021YFC2301005)。

摘　　要：目的了解GII.8和GII.9型诺如病毒P蛋白的糖结合特征。方法利用大肠杆菌系统获得GII.8和GII.9诺如病毒P颗粒蛋白,通过寡糖和唾液结合实验,研究P颗粒蛋白与组织血型抗原等糖的相互作用,再采用序列比对和结构比较,分析GII.8和GII.9糖结合位点的序列和结构特征。结果GII.8 P蛋白与所检测的寡糖无明显结合,与部分人A/B/O型别唾液样本有结合。GII.9 P蛋白结合H双糖,与人A/B/O型别唾液有很好结合。序列和结构分析均显示GII.8 P蛋白具有与GIII.9相似的潜在糖受体结合位点,其位点与流行株GII.4等型别的糖受体区区相近。结论非流行株GII.8和GII.9 P蛋白与不同组织血型抗原的结合能力不同,提示可能会造成流行的差异,需要对非流行株开展持续的分子监测。Objective To investigate the glycan binding specificity of the P protein in GII.8 and GII.9 noroviruses.Methods The P particle protein of GII.8 and GII.9 noroviruses was expressed by the Escherichia coli system.The oligosaccharide binding assay and saliva binding assay were carried out to explore the interactions between P particles and the histo-blood group antigens(HBGAs).Sequence alignment and structural superimposition were used to analyze the characteristics of the sequence and structure of the glycan binding sites.Results The P protein of GII.8 showed no obvious binding to the tested glycans while it bound to some saliva samples of A/B/O types.The P protein of GII.9 recognized H disaccharide and interacted with most of the saliva samples.The P protein of GII.8 possessed the similar potential glycan binding site as that of GII.9 according to the sequence and structural analysis.The site was close to that of prevalent GII.4 strains.Conclusions The non-dominant strains,GII.8 and GII.9 strains showed different glycan binding ability to different HBGAs,indicating that there may be discrepancy in prevalence.It is necessary to continually monitor the prevalence of non-dominant noroviruses.

关 键 词：诺如病毒 P颗粒蛋白 组织血型抗原 寡糖结合 唾液结合 

分 类 号：R373[医药卫生—病原生物学]