基于网络药理学的巴特日-7对输卵管炎大鼠核因子-κB p65的影响研究  

Effect of Batari-7 on nuclear factor-κB p65 in salpingitis rats based on network pharmacology

作　　者：玉荣[1] 徐艳华[2] 根玉[1] 韩志强[2] 巴图德力根[2] 舍力木格 YU Rong;XU Yan-hua;GEN Yu;HAN Zhi-qiang;BATU De-li-gen;SHELI Mu-ge(Mongolian Medicine of Department of Gynaecology,Affiliated Hospital of Inner Mongolia University for Nationalities,Tongliao 028007,Inner Mongolia,China;Institute of Mongolian Medicine Pharmacology,Affiliated Hospital of Inner Mongolia University for Nationalities,Tongliao 028007,Inner Mongolia,China)

机构地区：[1]内蒙古民族大学附属医院蒙医妇科,内蒙古通辽028007 [2]内蒙古民族大学附属医院蒙药药理研究所,内蒙古通辽028007

出　　处：《中国临床药理学杂志》2023年第6期881-886,共6页The Chinese Journal of Clinical Pharmacology

基　　金：内蒙古自治区自然科学基金资助项目(2020LH08010);内蒙古民族大学科学研究基金资助项目(NMDYB18088)。

摘　　要：目的筛选巴特日-7对输卵管炎大鼠的靶点蛋白并验证对核因子κB(NF-κB p65)的调控作用,研究巴特日-7对输卵管炎大鼠NF-κB p65的影响。方法运用网络药理学方法和TCMSP等数据库获得巴特日-7药物活性化合物,通过GeneCard等疾病靶点网站搜集输卵管炎潜在靶点,蛋白-蛋白相互作用网络分析。采用混合菌株接种法建立慢性输卵管炎大鼠模型,以验证巴特日-7对靶点蛋白的影响。将大鼠分空白对照组、模型组、阳性对照组、实验组,每组10只。除空白对照组外,其余各组均在子宫角-输卵管处注射混合菌液20μL,建立慢性输卵管炎模型。阳性对照组灌胃0.4 g·kg^(-1)金刚藤胶囊混悬液,实验组灌胃0.5 g·kg^(-1)巴特日-7混悬液,空白对照组和模型组灌胃蒸馏水,每日1次,各组均连续给药4周。用免疫组化法(IHC)检测输卵管-子宫角组织NF-κB p65蛋白表达。结果通过网络药理学手段筛选得到巴特日-7作用于输卵管炎的NF-κB、RIG-I样受体、Toll样受体等10个信号通路。进行基因本体(GO)和京都基因与基因组百科全书(KEGG)通路分析,共获得604个GO条目。空白对照组、模型组、阳性对照组、实验组NF-κB p65表达值分别为0.48±0.16、2.24±2.16、0.42±0.22、0.35±0.26,模型组较空白对照组上调,阳性对照组和实验组均较模型组下调,差异均有统计学意义(均P<0.05)。结论巴特日-7通过下调NF-κB p65蛋白表达,阻断NF-κB信号转导通路,从而抑制输卵管炎的形成。Objective To screen the target protein of Batari-7 on salpingitis rats,verify the regulation of nuclear factor-κB p65(NF-κB p65)and study the effect of Batari-7 on NF-κB p65 in salpingitis rats.Methods The active compounds of Batari-7 were obtained from TCMSP and other databases.Potential targets of salpingitis were collected from GeneCard and other disease target websites,and the protein-protein interaction network was analyzed.The rat model of chronic salpingitis was established by inoculation of mixed strains,and the effect of Batari-7 on the target protein was verified.The rats were divided into blank control group,model group,positive control group and experimental group,with 10 rats in each group.Except for the blank control group,the other groups were injected mixed bacterial solution 20μL into the uterine horn and oviduct outlet to establish the chronic salpingitis model.The positive control group was given 0.4 g·kg^(-1)Jinggang teng capsule suspension by gavage,the experimental group was given 0.5 g·kg^(-1)Batari-7 suspension by gavage,the blank control group and the model group were given the same volume of distilled water by gavage,once a day,for 4 weeks.Immunohistochemistry(IHC)was used to detect the expression of NF-κBP65 in oviduct-uterine horn tissues.Results Ten signaling pathways including NF-κB,RIG-I like receptor and Toll-like receptor in salpingotis were screened by network pharmacology.A total of 604 Gene Ontology(GO)items were obtained by GO and KEGG pathway analysis.The expression values of NF-κBP65 in blank control group,model group,positive control group and experimental group were 0.48±0.16,2.24±2.16,0.42±0.22 and 0.35±0.26,respectively.Compared with blank control group,the expression values of NF-κBP65 in positive control group and experimental group were up-regulated,and the differences were statistically significant(all P<0.05).Conclusion Batari-7 inhibits the formation of salpingitis by down-regulating NF-κB protein expression and blocking the NF-κB signal transductio

关 键 词：巴特日-7 输卵管炎 网络药理学 核因子ΚB 

分 类 号：R285[医药卫生—中药学]