B、T淋巴细胞衰减因子与系统性红斑狼疮患者疾病活动度及自身抗体的相关性分析  

Correlation analysis between B and T lymphocyte attenuator and disease activity and autoantibodies in patients with systemic lupus erythematosus

作　　者：安沛欣 闻苗苗 AN Peixin;WEN Miaomiao(Laboratory Department,Xi'an Fifth Hospital,Xi'an 710082,China)

机构地区：[1]西安市第五医院检验科,陕西西安710082

出　　处：《临床医学研究与实践》2023年第11期94-97,共4页Clinical Research and Practice

摘　　要：目的分析B、T淋巴细胞衰减因子(BTLA)与系统性红斑狼疮(SLE)患者疾病活动度及自身抗体的相关性。方法纳入2019年5月至2021年5月西安市第五医院收治的90例SLE患者作为研究对象,根据SLE疾病活动度指数(SLEDAI)将其分为低活动度组(56例)和高活动度组(34例),再纳入同期入院体检健康者90例作为对照组。比较三组B、T细胞表面BTLA的表达情况。比较不同自身抗体SLE患者B、T淋巴细胞表面BTLA的表达情况。使用Spearman检验分析SLE患者B、T细胞表面BTLA与疾病活动度、抗核小体抗体(AnuA)、抗双链DNA(anti-dsDNA)抗体的相关性。结果高活动度组的CD19^(+)/BTLA、CD24^(+)/BTLA、CD4^(+)/BTLA、CD8^(+)/BTLA均明显低于低活动度组和对照组,差异具有统计学意义(P<0.05);低活动度组的CD19^(+)/BTLA、CD24^(+)/BTLA、CD4^(+)/BTLA、CD8^(+)/BTLA均明显低于对照组,差异具有统计学意义(P<0.05)。90例SLE患者中,AnuA阳性43例,阴性47例,阳性率为47.78%;anti-dsDNA抗体阳性49例,阴性41例,阳性率为54.44%。AnuA、anti-dsDNA抗体阳性SLE患者的CD19^(+)/BTLA、CD24^(+)/BTLA、CD4^(+)/BTLA、CD8^(+)/BTLA均明显低于阴性患者,差异具有统计学意义(P<0.05)。SLE患者CD19^(+)/BTLA、CD24^(+)/BTLA、CD4^(+)/BTLA、CD8^(+)/BTLA与疾病活动度、AnuA、anti-dsDNA抗体均呈负相关(P<0.05)。结论SLE患者B、T细胞表面BTLA表达下调,且与疾病活动度、AnuA、anti-dsDNA抗体呈负相关。Objective To analyze the correlation between B and T lymphocyte attenuator(BTLA)and disease activity and autoantibodies in patients with systemic lupus erythematosus(SLE).Methods A total of 90 SLE patients admitted in Xi'an Fifth Hospital from May 2019 to May 2021 were included as the study objects.According to the SLE disease activity index(SLEDAI),the patients were divided into low activity group(56 cases)and high activity group(34 cases),and 90 healthy people undergoing physical examinees admitted in the hospital during the same period were included as control group.The expression of BTLA on the surface of B and T cells in the three groups was compared.The expression of BTLA on B and T lymphocytes in SLE patients with different autoantibodies were compared.Spearman test was used to analyze the correlation between BTLA on B and T cells and disease activity,anti-nucleosome antibody(AnuA)and anti-double stranded DNA antibody(anti-dsDNA)in SLE patients.Results The levels of CD19^(+)/BTLA,CD24^(+)/BTLA,CD4^(+)/BTLA and CD8^(+)/BTLA in the high activity group were significantly lower than those in the low activity group and the control group,and the differences were statistically significant(P<0.05);the levels of CD19^(+)/BTLA,CD24^(+)/BTLA,CD4^(+)/BTLA and CD8^(+)/BTLA in the low activity group were significantly lower than those in the control group,and the differences were statistically significant(P<0.05).Among 90 SLE patients,AnuA was positive in 43 cases and negative in 47 cases,with a positive rate of 47.78%;49 cases were anti-dsDNA antibody positive,41 cases were negative,and the positive rate was 54.44%.The levels of CD19^(+)/BTLA,CD24^(+)/BTLA,CD4^(+)/BTLA and CD8^(+)/BTLA in SLE patients with positive AnuA and anti-dsDNA antibody were significantly lower than those in negative patients,and the differences were statistically significant(P<0.05).The levels of CD19^(+)/BTLA,CD24^(+)/BTLA,CD4^(+)/BTLA and CD8^(+)/BTLA in SLE patients were negatively correlated with disease activity,AnuA and anti-dsDNA antibod

关 键 词：B、T淋巴细胞衰减因子 负向共刺激因子 系统性红斑狼疮 疾病活动度 自身抗体 

分 类 号：R392.1[医药卫生—免疫学]