梅毒患者血清IL-1β、IL-8表达水平与血清反应素滴度的相关性分析  

Correlation of serum IL-1βand IL-8 expression levels with serum reagin titer in patients with syphilis

作　　者：邓毅[1] 雷鸣[1] 吴雪峰[1] 赵飞骏[2] DENG Yi;LEI Ming;WU Xue-feng;ZHAO Fei-jun(The First People's Hospital of Changde City,Changde,Hunan 415003,China;Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control,Institute of Pathogenic Biology,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]常德市第一人民医院,湖南常德415003 [2]南华大学病原生物学研究所/特殊病原体防控湖南省重点实验室,湖南衡阳421001

出　　处：《实用预防医学》2023年第3期279-283,共5页Practical Preventive Medicine

基　　金：国家自然科学基金资助项目(81971980);湖南省出生缺陷协同防治重大专项子课题(2019SK1010)。

摘　　要：目的探讨不同临床分期梅毒患者血清白介素-1β(interleukin-1β,IL-1β)和白介素-8(interleukin-8,IL-8)表达水平与梅毒血清反应素滴度的相关性。方法收集40例健康体检者血清、120例梅毒患者血清(分为一期梅毒组、二期梅毒组和隐性梅毒组),采用酶联免疫吸附试验检测各组血清中IL-1β、IL-8水平,比较各组间IL-1β和IL-8表达水平的差异,采用梅毒甲苯胺红不加热血清试验(toluidine red unheated serum test,TRUST)检测梅毒患者血清反应素滴度,根据TRUST滴度将梅毒患者分为高滴度组和低滴度组,比较各组间IL-1β和IL-8表达水平的差异。结果各组血清IL-1β、IL-8表达水平差异有统计学意义(P<0.05),其中二期梅毒组血清IL-1β[15.10(10.63,19.16)]pg/ml和IL-8[108.67(69.96,139.79)]pg/ml明显高于一期梅毒组、隐性梅毒组和正常组;高滴度组IL-8[86.03(62.25,144.94)]pg/ml明显高于低滴度组[57.21(52.16,86.13)]pg/ml,差异有统计学意义(P<0.05),但高滴度组IL-1β[11.12(7.18,15.96)]pg/ml与低滴度组[8.43(6.93,14.05)]pg/ml比较差异无统计学意义(P>0.05);二期梅毒患者血清IL-8水平与反应素滴度呈正相关(r=0.625,P<0.05)。结论梅毒患者血清IL-1β和IL-8在一期梅毒、二期梅毒和隐性患者中均呈高水平表达,且IL-1β和血清IL-8表达水平变化与梅毒的病程进展有一定的相关性。Objective To explore the correlation between the expression levels of serum interleukin-1β(IL-1β)and interleukin-8(IL-8)and serum reagin titer in patients with syphilis at different clinical stages.Methods Serum samples were collected from 40 physical examinees and 120 patients with syphilis(who were divided into the primary syphilis group,the secondary syphilis group and the latent syphilis group).The serum levels of IL-1βand IL-8 in each group were measured by enzyme-linked immunosorbent assay.The toluidine red unheated serum test(TRUST)was used to test serum reagin titers of the syphilis patients.According to the TRUST titers,the syphilis patients were separated into the high titer group and the low titer group,and the differences in the expression levels of IL-1βand IL-8 between the groups were compared.Results The expression levels of serum IL-1βand IL-8 were significantly different among the groups(P<0.05),of which serum IL-1β(15.10(10.63,19.16))pg/ml and IL-8(108.67(69.96,139.79))pg/ml were significantly higher in the secondary syphilis group than in the primary syphilis group,the latent syphilis group and the normal group.Serum IL-8(86.03(62.25,144.94))pg/ml was significantly higher in the high titer group than in the low titer group(57.21(52.16,86.13))pg/ml,and the difference was statistically significant(P<0.05).However,no statistically significant difference was found in IL-1βbetween the high titer group(11.12(7.18,15.96)pg/ml)and the low titer group(8.43(6.93,14.05)pg/ml)(P>0.05).There was a positive correlation between serum IL-8 level and reagin titer in patients with secondary syphilis(r=0.625,P<0.05).Conclusion Serum IL-1βand IL-8 were expressed at high levels in patients with primary,secondary and latent syphilis,and there was a certain correlation between changes in serum levels of IL-1βand IL-8 expression and the disease progression of syphilis.

关 键 词：梅毒 白介素-1Β 白介素-8 甲苯胺红不加热血清试验 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]