甲基紫三波长叠加共振光散射法测定酒石酸泰乐菌素的含量  

Determination of tylosin tartrate by three-wavelength superimposed resonant light scattering method with methyl violet

作　　者：袁莉 刘毅 储文 袁嘉怡 马卫兴 YUAN Li;LIU Yi;CHU Wen;YUAN Jia-yi;MA Wei-xing(School of Pharmacy,Jiangsu Ocean University,Lianyungang 222005,China;School of Biomedical Industry,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Provincial Key Laboratory of Marine Drug Screening,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学药学院,江苏连云港222005 [2]江苏海洋大学生物医药产业学院,江苏连云港222005 [3]江苏省海洋药物筛选重点实验室,江苏连云港222005

出　　处：《现代化工》2023年第3期235-239,共5页Modern Chemical Industry

基　　金：江苏省品牌专业二期工程(省特色专业-药物制剂,编号65);2021年江苏省首批一流本科课程(线下一流课程-药物分析,序号104)。

摘　　要：在Clark-Lubs缓冲溶液中,基于酒石酸泰乐菌素与阳离子甲基紫试剂发生电荷转移反应,建立了三波长叠加共振光散射法(TRLS)测定酒石酸泰乐菌素含量的新方法。研究发现,在实验条件下,共振光散射图谱中会产生3个特征散射峰,分别在322、510、628 nm。在322、510、628 nm处,酒石酸泰乐菌素的质量浓度都在0.04~0.2 mg/L内呈线性关系;相关系数分别为0.999 4、0.999 5、0.999 2;检出限分别为0.036、0.040、0.038 mg/L。而采用三波长叠加法测定时,酒石酸泰乐菌素的质量浓度在0.04~0.2 mg/L内呈线性关系,相关系数为0.999 9,检出限为0.013 mg/L,检出限为单波长检测法的3倍。因建立的TRLS新方法简便、省时、灵敏度高,故可用于酒石酸泰乐菌素含量的大批量检测。In Clark-Lubs buffer solution, a novel three-wavelength superimposed resonance light scattering(TRLS) method for measuring the content of tylosin tartrate is established based on the charge transfer reaction between tylosin tartrate and cationic methyl violet reagent.It is found that under experimental conditions, three characteristic scattering peaks will appear in the resonance light scattering spectrum at 322 nm, 520 nm and 628 nm, respectively.At 322 nm, 510 nm, and 628 nm, the mass concentration of tylosin tartrate shows linear relationship within 0.04-0.2 mg·L^(-1),with correlation coefficients of 0.999 4,0.999 5,and 0.999 2,respectively, and detection limits of 0.036 mg·L^(-1),0.040 mg·L^(-1)and 0.038 mg·L^(-1),respectively when single-wavelength detection method is employed.When three-wavelength superimposed method is employed, the mass concentration of tylosin tartrate shows a linear relationship within the range of 0.04-0.2 mg·L^(-1),with a correlation coefficient of 0.999 9,and a detection limit of 0.013 mg·L^(-1)that is one third that by single-wavelength detection method.The newly established TRLS method is simple, time-saving and highly sensitive, so it can be used for mass detection of tylosin tartrate content.

关 键 词：酒石酸泰乐菌素 甲基紫(MV) 三波长叠加共振光散射法(TRLS) 电荷转移反应 

分 类 号：O657.39[理学—分析化学]