雄性大鼠四肢骨生物力学性能增龄性变化  

Age-Related Changes of Biomechanical Properties for Limb Bones in Male Rats

作　　者：杨林[1] 李曼 黄江[1] 王杰[1] 夏冰[1] 丁九阳 乐翠云[1] 李明杰 李正东 汪家文[1] YANG Lin;LI Man;HUANG Jiang;WANG Jie;XIA Bing;DING Jiuyang;LE Cuiyun;LI Mingjie;LI Zhengdong;WANG Jiawen(School of Forensic Medicine,Guizhou Medical University,Guiyang 550004,China;Department of Pathology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;Shanghai Key Laboratory of Forensic Medicine,Shanghai Forensic Service Platform,Academy of Forensic Science,Ministry of Justice,Shanghai 200063,China)

机构地区：[1]贵州医科大学法医学院,贵阳550004 [2]广西医科大学第一附属医院病理科,南宁530021 [3]司法鉴定科学研究院上海市法医学重点实验室,司法部司法鉴定重点实验室,上海市司法鉴定专业技术服务平台,上海200063

出　　处：《医用生物力学》2023年第1期170-175,共6页Journal of Medical Biomechanics

基　　金：贵州省科技厅基金项目(黔科合平台人才[2018]5779-3);贵州省科技支撑项目(黔科合支撑[2022]一般280)。

摘　　要：目的探讨雄性大鼠肱骨、股骨和胫骨生物力学性能增龄性变化规律及其在年龄推断中的应用价值。方法根据不同周龄,将90只健康雄性SD大鼠分为2、4、6、8、17、26、52、78和104周龄组,每组10只。过量麻醉处死大鼠后分离肱骨、股骨和胫骨并剔除附着软组织,利用游标卡尺测量上述骨骼长度及中段(受压部位)直径,利用电子万能材料试验机进行三点弯曲试验以检测极限载荷和极限载荷下位移。结果不同周龄组雄性大鼠肱骨、股骨和胫骨极限载荷组间差异均具有统计学意义(P<0.05)。随着周龄增长,肱骨、股骨和胫骨极限载荷均呈先增大后减小趋势,且都在52周龄组达峰值,52周龄前与周龄呈强正相关(r=0.884、0.933、0.929,P<0.05)。极限载荷下位移除股骨组间差异有统计学意义外(P<0.05),肱骨、胫骨均无统计学意义。股骨极限载荷下位移与周龄呈弱正相关(r=0.406,P<0.05)。52周龄前大鼠肱骨、股骨、胫骨、3种骨骼综合极限载荷自动线性建模的年龄预测准确度分别为78.2%、86.8%、84.1%、88.3%。肱骨、股骨、胫骨长度与极限载荷呈强正相关(r=0.904、0.897、0.814,P<0.05)。肱骨、股骨、胫骨中段直径与极限载荷呈强正相关(r=0.759、0.814、0.745,P<0.05)。结论雄性大鼠肱骨、股骨和胫骨极限载荷随周龄增长先增大后减小,在52周龄前与周龄呈正相关,可用于年龄推断。3种骨骼极限载荷综合推断年龄准确度最高,其次为股骨。肱骨、股骨、胫骨长度/中段直径均与极限载荷呈强正相关。Objective To investigate the age-related changes of biomechanical properties for humerus,femur and tibia in male rats and their application values in age estimation.Methods According to different weeks of age,90 healthy male SD rats were divided into 2,4,6,8,17,26,52,78 and 104-week groups with 10 rats in each group.After the rats were executed by excessive anesthesia,humerus,femur,and tibia were separated and the attached soft tissues were removed.The length of the above-mentioned bones and the diameter of the middle section(compression site)were measured with vernier caliper,and the three-point bending test was conducted with electronic universal material testing machine to detect the ultimate load and displacement under ultimate load.Results There were significant differences in the ultimate load of humerus,femur and tibia among male rats in different age groups(P<0.05).With the increase of week age,the ultimate loads of the humerus,femur and tibia increased first and then decreased,and reached the peak value in 52-week age group,showing a strong positive correlation with week age before 52 weeks(r=0.884,0.933,0.929,P<0.05).There was no significant difference in humerus and tibia.The displacement of femur under ultimate load was weakly positively correlated with week age(R=0.406,P<0.05).The age prediction accuracy for automatic linear modeling of ultimate load for humerus,femur,tibia and three above-mentioned bones in rats before 52-week age was 78.2%,86.8%,84.1%and 88.3%,respectively.There was a strong positive correlation between the length of humerus,femur and tibia and the ultimate load(R=0.904,0.897,0.814,P<0.05).The diameters of humerus,femur and tibia were strongly positively correlated with the ultimate load(R=0.759,0.814 and 0.745,P<0.05).Conclusions The ultimate loads of humerus,femur and tibia in male rats increased first and then decreased with age,and were positively correlated with age before 52 weeks,which could be used for age inference.The highest accuracy of age estimation was ultimate loads

关 键 词：大鼠 肱骨 股骨 胫骨 生物力学 年龄推断 

分 类 号：R318.01[医药卫生—生物医学工程]