海洋生境间苯三酚高产菌株选育及其培养基优化  

Breedinghigh phloroglucinol and optimization of medium

作　　者：马湘君 王祥法 侯仕营 李银塔 田黎 MA Xiangjun;WANG Xiangfa;HOU Shiying;LI Yinta;TIAN Li(Weihai Ocean Vocational College,Rongcheng,Shandong 264300;Marine Economic Algae Resource Development and Utilization Engineering Technology Collaborative Innovation Center of Shandong Province,Rongcheng,Shandong 264300;The First Institute of Oceanography,Qingdao 266100)

机构地区：[1]威海海洋职业学院,山东荣成264300 [2]山东省海洋经济藻类资源开发与利用工程技术协同创新中心,山东荣成264300 [3]自然资源部第一海洋研究所,山东青岛266100

出　　处：《海洋湖沼通报》2023年第1期67-73,共7页Transactions of Oceanology and Limnology

基　　金：山东省海洋经济藻类资源开发与利用工程技术协同创新中心科研开放基金项目(MADU 202001,MADU20210001);威海海洋职业学院水产养殖技术高水平专业群项目资金支持;2020年山东省职业教育技艺技能传承创新平台建设计划(96);潍坊市2021年科技发展计划项目(2021GX026)。

摘　　要：本研究以一株分离筛选到的产间苯三酚的海洋生境假单胞菌LBF-96为基础,通过传统紫外诱变以及紫外微波复合诱变选育间苯三酚高产菌株,并采用响应曲面法优化突变株的发酵培养基。结果显示:经紫外照射60 s、微波辐照20 s复合诱变获得了一株间苯三酚高产菌YLM96-01,利用肉桂醛显色法测得其间苯三酚产量为0.599 g/L,较原始菌株提高了159%,且产间苯三酚时间提前,降低了发酵培养的成本;经传代培养显示突变株YLM96-01具有良好的遗传稳定性。采用响应曲面法优化培养条件后,突变株YLM96-01间苯三酚的产量提高为0.784 g/L,比优化前提高31%。通过紫外微波复合诱变选育出的间苯三酚高产菌YLM96-01,具有潜在生产应用价值。his studymarine Pseudomonas LBF-96 isolated earlierphloroglucinol,and the fermentation medium of the mutant was optimized.UV for 60 s and microwave irradiation for 20 s.The yield of phloroglucinol 0.599 g/L cinnamaldehyde colorimetry,159% higher than that of the original.The production time of phloroglucinol was advanced and the cost of fermentation was reduced.he mutant YLM96-01 showed good genetic stabilitysubculture.After the culture condition w optimized response surface methodology,the yield of phloroglucinol mutant YLM96-01 increased to 0.784 g/L,31% higher than that.The high phloroglucinol-producing strain YLM96-01 bred potentialproduction.

关 键 词：间苯三酚 假单胞菌 紫外微波复合诱变 响应面法 

分 类 号：Q93[生物学—微生物学]