莱州湾海洋PAHs污染调查与生物监测技术的研究  

Research on marine PAHs pollution investigation and biological monitoring technology in Laizhou Bay

作　　者：曹云浩 潘鲁青[1] 孙家伟 李泽远 CAO Yunhao;PAN Luqing;SUN Jiawei;LI Zeyuan(Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《海洋湖沼通报》2023年第1期107-116,共10页Transactions of Oceanology and Limnology

基　　金：山东省重点研发计划项目(2018GHY115007)。

摘　　要：本文在2018年3、5、8、10月份开展了莱州湾PAHs污染调查和生物监测技术研究。结果表明:莱州湾表层沉积物ΣPAHs范围为197.53~575.73 ng/g d.w.,最低为S4站3月份,最高为S2站10月份;四角蛤蜊软体部为73.43~156.39 ng/g d.w.,最低为S4站3月份,最高为S2站8月份。表层沉积物、四角蛤蜊软体部中PAHs含量均值均呈现S2>S3>S1>S4的变化趋势。通过皮尔逊相关性分析得出鳃丝EROD、SOD、GPx、DNA损伤、LPO和消化盲囊CYP450、EROD、UGT、T-AOC、GPx、DNA损伤、PC、LPO可作为指示莱州湾PAHs污染的现场生物标志物。鳃丝与消化盲囊的IBR分析结果存在差异,鳃丝IBR得分均值呈现S2>S3>S4>S1的趋势,而消化盲囊则为S2>S3>S1>S4。MPI表明S2处于严重污染等级,S3处于轻度污染等级,S1与S4相近处于轻度与良好等级之间,鳃丝、消化盲囊的IBR和MPI结果表现出良好的一致性。其中消化盲囊IBR站点得分均值排序与化学监测结果排序相同,且消化盲囊的指数评价结果与莱州湾沉积物的PAHs含量显著相关。由此可见,综合生物指数分析方法是评估海洋环境污染状况的有效工具。This study carried out the PAHs pollution investigation and biological monitoring technology research in Laizhou Bay in March,May,August and October 2018.The results showed that the surface sediment ΣPAHs in Laizhou Bay ranged from 197.53 to 575.73 ng/g dw,the lowest was S4 site in March,and the highest was S2 site.October;the four-corner clam software department is 73.43-156.39 ng/g dw,the lowest is S4 site in March,and the highest is S2 site in August.The average value of PAHs in the surface sediments and the soft parts of the four-cornered clams showed a trend of S2 S3 S1 S4.Pearson correlation analysis showed that gill's EROD,SOD,GPx,DNA damage,LPO and digestive glands' CYP450,EROD,UGT,T-AOC,GPx,DNA damage,PC,LPO can be used as indicators of PAHs pollution in Laizhou Bay.The results of IBR analysis of gill and digestive glands were different.The average gill's IBR scores show a trend of S2 S3 S4 S1,while the digestive glands were S2 S3 S1 S4.MPI showed that S2 was in a serious pollution level,S3 was in a light pollution level,S1 and S4 were similar between light and good levels,and the IBR and MPI results of gill and digestive glands showed good consistency.Among them,the average ranking of the digestive glands' IBR scores was the same as the chemical monitoring results,and the index evaluation results of the digestive glands were significantly related to the PAHs content in the sediments of Laizhou Bay.Thus,the comprehensive biological index analysis method is an effective tool for assessing the pollution of the marine environment.

关 键 词：莱州湾 多环芳烃 四角蛤蜊 生物标志物 综合生物指数 

分 类 号：S917.4[农业科学—水产科学]