经皮浅表超声造影动态观察兔乳腺癌淋巴转移模型中前哨淋巴结的变化  

The Dynamic Observation of Sentinel Lymph Nodes by Percutaneous Contrast Enhanced Ultrasound in Rabbit Model of Lymphatic Metastasis of Breast Cancer

作　　者：王文 申洪远 张茜 杨光飞[3] 王福霞 米成嵘[3] Wang Wen;Shen Hongyuan;Zhang Qian;Yang Guangfei;Wang Fuxia;Mi Chengrong(Department of Ultrasound,Cardiovascular and Cerebrovascular Disease Hospital,General Hospital of Ningxia Medical University,Yinchuan 750002,China;Department of Ultrasound,Affiliated Zhongda Hospital of Southeast University,Nanjing 210009,China;Department of Ultrasound,General Hospital of Ningxia Medical University,Yinchuan 750003,China)

机构地区：[1]宁夏医科大学总医院心脑血管病医院超声科,银川市750002 [2]东南大学附属中大医院超声科,南京市210009 [3]宁夏医科大学总医院超声科,银川市750003

出　　处：《中国超声医学杂志》2023年第3期333-336,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金项目(No.81960317);宁夏自然科学基金项目(No.2021AAC03346)。

摘　　要：目的 通过兔乳腺癌淋巴转移模型动态观察前哨淋巴结(SLN)增强模式。方法 VX2(兔间变表皮鳞癌细胞株)组织悬液建立兔乳腺癌淋巴转移模型,隔天行二维超声及SLN示卓安超声造影(CEUS)检查,观察转移过程中SLN造影模式变化。结果 随着肿瘤进展,SLN增强模式也随之变化,表现为均匀性增强、非均匀性增强、边缘环状增强内部无增强及完全无增强淋巴结周围出现淋巴管绕行4种模式,其中非均匀性增强模式随时间表现为:a.无增强区域<25%;b.无增强区域25%~50%;c.无增强区域50%~75%;d.无增强区域>75%/完全不增强。结合病理结果,4种增强超声造影表现可有效评估SLN良恶性(P<0.05)。结论 借助兔乳腺癌淋巴转移模型,经皮示卓安浅表超声造影(SNZ-CEUS)可沿淋巴管精确定位及区分SLN,在乳腺癌转移过程中评估SLN状态、观察淋巴系统重建中具有重要价值。Objective To establish the lymph node metastasis model of breast cancer in rabbits, and to explore the enhancement change in lymph node metastasis by superficial contrast-enhanced ultrasound(CEUS). Methods The lymph node metastasis model of breast cancer in rabbit was established by using VX2 tissue suspension. 2D ultrasonography and CEUS of superficial sentinel lymph node(SLN) were performed regularly to observe the changes of SLN imaging patterns during the invasion of breast cancer. Results As tumor progressed, the enhancement pattern of SLN changed, showing four patterns as homogeneous enhancement, non-homogeneous enhancement, marginal circumferential enhancement without internal enhancement, and completely non-enhancing lymph nodes surrounded by lymphatic vascular bypass, where the non-homogeneous enhancement pattern showed: a. no enhancement area <25%;b. no enhancement area 25% to 50%;c. no enhancement area 50% to 75%;and d. no enhancement region >75%/no enhancement at all. Combined with pathological findings, the four enhanced ultrasonographic presentations could effectively assess the benignity and malignancy of SLN(P<0.05). Conclusions With the help of VX2 rabbit model of lymphatic metastasis of breast cancer, the superficial Sonazoid CEUS(SNZ-CEUS) can accurately locate and differentiate SLN along lymphatic vessels, which is of great value in assessing SLN status and observing lymphatic system reconstruction during breast cancer metastasis.

关 键 词：乳腺癌 淋巴转移 前哨淋巴结 VX2动物模型 浅表超声造影 

分 类 号：R445.1[医药卫生—影像医学与核医学] R737.9[医药卫生—诊断学]