小麦ABF/AREB/ABI5基因家族全基因组鉴定与表达分析  

Genome-wide Identification and Expression Analysis of ABF/AREB/ABI5 Gene Family in Wheat(Triticum aestivum)

作　　者：郭树娟 孙月 郑昊元 赵惠贤[1] 马猛[1] 刘香利[1] GUO Shu-Juan;SUN Yue;ZHENG Hao-Yuan;ZHAO Hui-Xian;MA Meng;LIU Xiang-Li(College of Life Science,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学生命科学学院,杨陵712100

出　　处：《农业生物技术学报》2023年第4期667-681,共15页Journal of Agricultural Biotechnology

基　　金：陕西省重点研发计划项目(2021NY-079);杨凌示范区产学研用协同创新重大项目(2017CXY-01)。

摘　　要：脱落酸响应元件结合因子/脱落酸响应元件结合蛋白/脱落酸不敏感蛋白5(abscisic acid(ABA)-responsive element binding factors/ABA-responsive element binding proteins/ABA insensitive 5,ABF/AREB/ABI5)属于碱性亮氨酸拉链(basic leucine zipper,bZIP)转录因子A亚家族,参与植物ABA信号通路,调控下游基因表达,进而影响植物生长发育、逆境胁迫响应等。本研究对小麦(Triticum aestivum)ABF/AREB/ABI5基因进行了全基因组鉴定,并对其基因系统进化关系、理化性质、基因结构、基因复制、启动子顺式作用元件及表达模式进行了分析。在小麦全基因组范围内共鉴定到36个TaABF基因,分布在除了7B外的小麦所有染色体上,且在第Ⅲ部分同源群上存在串联复制和片段复制事件。TaABF编码区长度为672~1191 bp,蛋白等电点为5.22~9.93,均为亲水性蛋白。根据系统进化分析可分为3组,每组中的TaABF基因在结构和保守基序上表现出高度相似性。启动子顺式作用元件分析表明,TaABF基因家族启动子具有多种响应激素和非生物胁迫相关的顺式作用元件。转录组表达模式分析显示,不同TaABF基因呈现组织和发育时期差异性表达,多数基因在种子发育时期高表达;与拟南芥AREB/ABF亚家族同源的GroupⅠ中基因TaABF17、TaABF18、TaABF19在干旱和盐胁迫下均上调表达。进一步利用q PCR对TaABF17、TaABF18、TaABF19基因在ABA和干旱、盐处理下的表达进行分析,结果表明,ABA处理后TaABF18显著上调表达,而TaABF17和TaABF19显著下调表达;干旱和盐处理后TaABF17、TaABF18、TaABF19基因都显著上调表达。本研究为小麦TaABF基因抗逆功能研究提供了基础资料。Abscisic acid(ABA)responsive element binding factors/abscisic acid responsive element binding proteins/abscisic acid insensitive 5(ABF/AREB/ABI5)belongs to the basic leucine zipper(bZIP)transcription factors A subfamily,which participate in ABA signaling pathway by regulating the expression of downstream genes,and thereby affect the plant growth and development,as well as in response to abiotic stress.In this study,the members of the ABF/AREB/ABI5 subfamily were identified in the whole genome of wheat(Triticum aestivum),and the phylogenetic relationship,physical and chemical properties,gene structure,gene replication,cis-acting elements of promoters and the expression pattern of wheat TaABFs were analyzed.The results showed that a total of 36 TaABFs were identified in wheat,which were distributed on all chromosomes except 7B and there were tandem duplication and fragment duplication events on theⅢhomologous group.The coding region of wheat TaABF proteins were 672~1191 bp in length,and their isoelectric point were 5.22~9.93,all of which were hydrophilic proteins.According to phylogenetic analysis,TaABFs were divided into 3 groups,and in each group,the TaABFs showed high similarity in structure,conserved motifs and physicochemical properties.The promoter analysis of TaABFs indicated that there were a large number of cis-acting elements in response to hormones and abiotic stresses in their promoters.Transcriptome expression pattern analysis showed that the TaABF genes expressed differentially in different tissues and developmental stages,most of which were highly expressed during seed development.The TaABF genes also showed different responses to drought,salt and low temperature stress,and the TaABF genes in groupⅠhomologous to the Arabidopsis AREB/ABF subfamily were up-regulated under both drought and salt stress.Further expression analysis of TaABF genes in GroupⅠby qPCR showed that,under ABA treatment,TaABF18 was up-regulated,while TaABF17 and TaABF19 were significantly down-regulated.TaABF17,TaABF18 and Ta

关 键 词：小麦 ABF/AREB/ABI5亚家族 生物信息学分析 表达模式 

分 类 号：S512.1[农业科学—作物学]