棉花Gols基因家族的鉴定及其在干旱胁迫应答中的功能研究  

Identification of Gols Gene Family in Cotton(Gossypium spp.)and Its Function Analysis in Drought Stress Response

作　　者：古丽斯坦•赛米 刘隋赟昊 郑蓓 陈永坤 黄耿青 赵惠新[1] 张经博[1] SAIMI Gu-Li-Si-Tan;LIU Sui-Yun-Hao;ZHENG Bei;CHEN Yong-Kun;HUANG Geng-Qing;ZHAO Hui-Xin;ZHANG Jing-Bo(College of Life Sciences,Xinjiang Normal University,Urumqi 830054,China)

机构地区：[1]新疆师范大学生命科学学院,乌鲁木齐830054

出　　处：《农业生物技术学报》2023年第4期704-717,共14页Journal of Agricultural Biotechnology

基　　金：新疆维吾尔自治区自然科学基金(2022D01B39)。

摘　　要：棉子糖在植物应答非生物胁迫过程中发挥着重要的作用,肌醇半乳糖苷合成酶(galactinol synthase,Gols)是参与合成棉子糖的重要酶类。本研究在全基因水平上对Gols基因家族进行了鉴定和分析,在亚洲棉(Gossypium arboreum)和雷蒙德氏棉(G.raimondill)中分别鉴定出8个和7个Gols基因,在陆地棉(G.hirsutum)和海岛棉(G.barbadense)中均鉴定14个Gols基因。进化分析显示,Gols基因家族可分为7个亚组。保守基序和基因结构分析显示,亲缘关系近的Gols基因之间的保守基序和基因结构也十分相似。启动子顺式作用元件分析显示,GhGols基因存在多个与非生物胁迫应答相关的顺式作用元件。组织表达模式分析显示,一些GhGols基因在特定的组织中优势表达。PEG处理下的表达分析显示,部分GhGols基因的表达受到PEG的显著诱导,其中GhGols6的表达受PEG诱导显著上调。qPCR结果显示GhGols6的转录水平也受到干旱胁迫的显著诱导(P<0.01)。进一步利用病毒诱导的基因沉默技术(virus induced gene silencing,VIGS)对GhGols6基因进行沉默,发现沉默GhGols6降低了棉花对干旱胁迫的耐受性,表明GhGols6是棉花干旱应答的正调控因子。本研究为后续深入研究Gols基因在棉花干旱胁迫应答中的功能提供了参考。Raffinose plays an important role in plant response to abiotic stress,and galactinol synthase(Gols)is an important enzyme involved in the synthesis of raffinose.In this study,Gols gene families were identified and analyzed in the whole genome,8,7,14 and 14 Gols genes were identified in Gossypium arboreum,G.raimondi,G.hirsutum and G.barbadense,respectively.Evolutionary analysis showed that the Gols gene family could be divided into 7 subgroups.The resules of conservative motifs and gene structure analysis showed that gene structure and composition of conserved motifs were also similar between closely related family members.A lot of cis-elements relevant to plant response to stresses were distributed on promoter regions of GhGols.Tissue expression pattern analysis showed that some GhGols genes were predominantly expressed in specific tissues.Expression analysis showed that the expression of some GhGols was induced by PEG treatment,among which the expression of GhGols6 was significantly up-regulated under PEG treatment.The results of qPCR analysis showed that the transcription level of GhGols6 was also significantly induced under drought stress.Further the GhGols6 gene was silenced using virus induced gene silencing(VIGS)technology,the result showed that silencing GhGols6 decreased the tolerance of cotton to drought stress,indicating that GhGols6 was a positive regulator of cotton drought response.This study provides a reference for further study on the function of Gols gene in cotton drought response.

关 键 词：棉花 干旱胁迫 肌醇半乳糖苷合成酶(Gols) 表达分析 病毒诱导的基因沉默(VIGS) 

分 类 号：S188[农业科学—农业基础科学]