不同投加密度的霍甫水丝蚓对穗花狐尾藻化感抑藻效应的影响  

Effect of different density of Limnodrilus hoffmeisteri on allelopathic Myriophyllum spicatum inhibition

作　　者：杨浩 张青[1] 姚存静 张丽丽[1] 胡伟绩 左胜鹏[1] YANG Hao;ZHANG Qing;YAO Cunjing;ZHANG Lili;HU Weiji;ZUO Shengpeng(School of Ecology And Environment,Anhui Normal University,Wuhu241000,China)

机构地区：[1]安徽师范大学生态与环境学院,芜湖241000

出　　处：《环境工程学报》2023年第2期666-674,共9页Chinese Journal of Environmental Engineering

基　　金：2017年安徽省自然科学基金面上项目(511705);2021年本科生优秀毕业论文(设计、创作)培育计划项目(779)。

摘　　要：利用沉水植物释放化感物质抑制水体中蓝藻生长是目前生态安全性极高的抑藻手段。穗花狐尾藻已被证实具有抑藻作用,为此,从外在生物作用可能会对沉水植物抑藻产生影响的角度出发,选用霍甫水丝蚓和穗花狐尾藻、铜绿微囊藻(FACHB-524)为研究对象,以低、中、高密度霍甫水丝蚓与穗花狐尾藻进行共培养,同时设置对照组(无霍甫水丝蚓投加即零密度组),并以蒸馏水代替种植水为空白组;对穗花狐尾藻种植水进行抑藻效应测定,对比分析了各组铜绿微囊藻的藻密度、SOD酶活性、MDA含量、叶绿素-a含量的变化。结果表明:处理组的抑藻效应显著高于对照组,霍甫水丝蚓对穗花狐尾藻的促进作用与霍甫水丝蚓密度相关,且霍甫水丝蚓对穗花狐尾藻化感抑藻效应存在一个最佳密度。不同密度(低、中、高)的霍甫水丝蚓的存在均可增强穗花狐尾藻化感作用。其中中密度组最为显著,其抑藻率最高可达到62.22%,各处理组叶绿素-a含量下降,中密度组最为显著。对藻细胞的生理指标进行分析,发现处理组中SOD活性先升后降、MDA含量持续升高,化感物质造成藻细胞活性氧的过量积累可能是导致其死亡的直接原因。The release of allelopathic substances by submerged plants to inhibit the growth of cyanobacteria in waterbody is a highly ecological safety method. Myriophyllum spicatum has been proved to have an algal inhibiting effect. In this study, Limnodrilus hoffmeisteri, Myriophyllum spicatum and Microcystis aeruginosa(FACHB-524) were chosen as the main research objects from the perspective that external biological effects might affect the algal inhibiting effect of submerged plants. High, medium, and low densities of Limnodrilus hoffmeisteri and Myriophyllum spicatum were cocultured, and control 1(zero density group without the addition of Limnodrilus hoffmeisteri) and control 2(control group) with distilled water instead of planting water were set up in the experments. The algal inhibiting effect of Myriophyllum spicatum planting water was determined. The variations of density, SOD activity, MDA content and chlorophyll a content of Microcystis aeruginosa in each group were measured for comparative analysis. The results showed that the algal inhibiting effect of the treatment group was significantly higher than that of the control group. The promoting effect of the Limnodrilus hoffmeisteri on the Myriophyllum spicatum was related to the density of the Limnodrilus hoffmeisteri, and there was an optimal density of the allelopathic algal inhibiting effect of the Limnodrilus hoffmeisteri on the Myriophyllum spicatum. The allelopathy of Myriophyllum spicatum was enhanced by the presence of different densities(low, medium and high) of Limnodrilus hoffmeisteri. of which the medium density group had the most significant inhibiting effect with the highest algal inhibiting rate of 62.22%. The content of chlorophyll a decreased in all treatment groups, and the medium density group was the most one. The analysis of physiological indexes of algal cells showed that SOD activity increased firstly and then decreased, and MDA content continued to increase in the treatment group. The excessive accumulation of reactive oxygen species caus

关 键 词：霍甫水丝蚓 花狐尾藻 共培 铜绿微囊藻 化感抑藻效应 

分 类 号：X703[环境科学与工程—环境工程]