山柰酚对人表皮黑素细胞黑素合成及增殖凋亡的影响观察  

Effects of kaempferol on melanin synthesis,proliferation,and apoptosis of human epidermal melanocytes

作　　者：王红娟[1] 雷子贤 胡雯[1] 康晓静[1] WANG Hongjuan;LEI Zixian;HU Wen;KANG Xiaojing(Department of Dermatology and Venereology,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院皮肤性病科新疆皮肤病临床医学研究中心新疆皮肤病研究重点实验室,乌鲁木齐830001

出　　处：《山东医药》2023年第9期22-25,共4页Shandong Medical Journal

基　　金：新疆维吾尔自治区自然科学基金资助项目(2021D01C197)。

摘　　要：目的观察山柰酚对人表皮黑素细胞黑素合成及增殖凋亡的影响。方法取20岁以下男性包皮标本制作人表皮黑素细胞悬液,培养后采用CCK-8法确定山柰酚、H_(2)O_(2)的应用剂量。取黑素细胞分成10μmol/L山柰酚组、阴性对照组:10μmol/L山柰酚组加入10μmol/L的山柰酚,阴性对照组加入等体积培养液,采用NaOH裂解法观察山柰酚对人表皮黑素细胞黑素合成的影响,以黑素合成量表示。取黑素细胞分成对照组、山柰酚组、H_(2)O_(2)组、山柰酚+H_(2)O_(2)组:对照组加入黑素细胞培养48 h,山柰酚组加入10μmol/L山柰酚培养48 h,H_(2)O_(2)组加入0.8 mmol/L H_(2)O_(2)培养48 h,山柰酚+H_(2)O_(2)组加入10μmol/L山柰酚处理24 h后继续加入0.8 mmol/L H_(2)O_(2)再培养24 h;采用CCK-8法观察山柰酚对黑素细胞增殖的影响,以细胞增殖率表示;采用Annenxin V/PI双染色法观察山柰酚对黑素细胞凋亡的影响,以细胞凋亡率表示。结果阴性对照组、10μmol/L山柰酚组黑素合成量分别为100.33±0.68、162.97±3.34,两组相比,P<0.05。对照组、山柰酚组、H_(2)O_(2)组、山柰酚+H_(2)O_(2)组细胞增殖率分别为100.61%±1.71%、110.27%±5.83%、51.49%±2.35%、74.96%±0.53%,组间相比,P均<0.05。对照组、山柰酚组、H_(2)O_(2)组、山柰酚+H_(2)O_(2)组细胞凋亡率分别为4.30%±0.72%、3.80%±1.21%、25.93%±2.23%、9.73%±2.39%,其中H_(2)O_(2)组细胞凋亡率高于其余各组(P均<0.05);对照组、山柰酚组、山柰酚+H_(2)O_(2)组细胞凋亡率组间相比,P均>0.05。结论10μmol/L的山柰酚可促进人表皮黑素细胞的黑素合成。山柰酚预处理可促进H_(2)O_(2)刺激下的黑素细胞增殖,抑制其凋亡。Objective To observe the effects of kaempferol on melanin synthesis,proliferation,and apoptosis of human epidermal melanocytes.Methods Human epidermal melanocyte suspension was prepared from male prepuce samples under 20 years old,and the applied dose of kaempferol and H_(2)O_(2) was determined by CCK-8 method after culture.We took melanocytes and divided them into 10μmol/L kaempferol group and negative control group;10μmol/L kaempferol was added to the 10μmol/L kaempferol group,and the same volume of culture medium was added to the negative control group.The effect of kaempferol on melanin synthesis of human epidermal melanocytes was observed by NaOH lysis method(expressed by melanin synthesis).The melanocytes were divided into the control group,kaempferol group,H_(2)O_(2) group,and kaempferol+H_(2)O_(2) group:the melanocytes in the control group were cultured for 48 h with melanocytes,and the melanocytes in the kaempferol group were cultured for 48 h with 10μmol/L kaempferol,the melanocytes in the H_(2)O_(2) group were cultured for 48 h with 0.8 mmol/L H_(2)O_(2),and the melanocytes in the Kaempferol+H_(2)O_(2) group were first treated with 10μmol/L kaempferol for 24 h,and then were cultured for 24 h with 0.8 mmol/L H_(2)O_(2).CCK-8 method was used to observe the effect of kaempferol on melanocyte proliferation(expressed by cell proliferation rate);the effect of kaempferol on the apoptosis of melanocytes was observed by annexinV/PI double staining method(expressed by the apoptosis rate).Re⁃sults The amount of melanin synthesis in the negative control group and 10μmol/L kaempferol group was 100.33±0.68 and 162.97±3.34,respectively,with statistically significant difference between the two groups(P<0.05).The cell proliferation rates of the control group,kaempferol group,H_(2)O_(2) group and kaempferol+H_(2)O_(2) group were 100.61%±1.71%,110.27%±5.83%,51.49%±2.35%and 74.96%±0.53%,respectively,with statistically significant difference between groups(all P<0.05).The apoptosis rates of the control group,

关 键 词：山柰酚 黑素细胞 黑色素 氧化应激 H_(2)O_(2) 白癜风 

分 类 号：R758.4[医药卫生—皮肤病学与性病学]