基于OD值和撞击式空气微生物采样器的空气消毒效果鉴定方法  

Evaluation method for air disinfection effect based on OD value and six-stage air sampler

作　　者：杨修利 陈敏 姚青 郭国良 陈猛 郑军妹 YANG Xiu-li;CHEN Min;YAO Qing;GUO Guo-liang;CHEN Meng;ZHENG Jun-mei(Ningbo Fotile Kitchen Ware Co.,Ltd.,Zhejiang 315336,China;不详)

机构地区：[1]宁波方太厨具有限公司,浙江315336 [2]浙江省健康智慧厨房系统集成重点实验室

出　　处：《中国卫生检验杂志》2023年第5期550-553,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的 在评价空气消毒效果鉴定试验中,研究气雾室内空气初始微生物浓度的控制方法。方法 以白色葡萄球菌为试验菌,通过可见分光光度计和六级筛孔撞击式空气微生物采样法,对气雾室喷雾染菌浓度进行分析和优化。结果 选用OD600值为0.012~0.051的白色葡萄球菌菌悬液进行染菌,并将采样时间控制在5 s~10 s,气雾室内白色葡萄球菌浓度可达到1.51×10^(5)cfu/m^(3)~3.59×10^(5)cfu/m^(3),符合空气消毒模拟现场试验标准要求,且气雾室内最终的白色葡萄球菌浓度与菌悬液OD600值成线性关系(r=0.996 8),基于该线性关系,可以更好地实现气雾室白色葡萄球菌浓度的精准控制。结论 该方法将气雾室内空气初始微生物浓度稳定控制在要求范围内,为准确评价空气净化除菌性能试验效果提供借鉴意义。Objective In the evaluation test of air disinfection effect, the control method of initial air microbial concentration in aerosol chamber was studied. Methods With Staphylococcus albicans as the test bacteria, the concentration of spray contamination in the aerosol chamber was analyzed and optimized through a visible spectrophotometer and a six-stage air sampler. Results Staphylococcus albicans bacterial suspension with OD600value of 0.012-0.051 was selected for contamination, and the sampling time was controlled within 5-10 seconds, the concentration of Staphylococcus albicans in the aerosol chamber can reach 1.51×10~5 cfu/m~3-3.59×10~5 cfu/m~3, which meets the requirements of the air disinfection simulation field test standard, and the final Staphylococcus albicans concentration in the aerosol room has a linear relationship with the OD600value of the bacterial suspension(r=0.996 8). Based on this linear relationship, the precise control of the concentration of Staphylococcus albicans in the aerosol chamber can be better achieved. Conclusion The method can stably control the initial microbial concentration of the air in the aerosol chamber within the required range, and provide a reference for the accurate evaluation of the test effect of air purification and sterilization performance.

关 键 词：OD值 六级筛孔撞击式空气微生物采样器 白色葡萄球菌 空气消毒效果 模拟现场试验 

分 类 号：R446.5[医药卫生—诊断学]