MEBO对高糖培养的成纤维细胞外泌体分泌的影响  

Effect of MEBO on the secretion of exosomes in fibroblasts cultured with high glucose

作　　者：周安邦 徐媛媛 郭小英 周宇 代平平 农丝洁 吴标良[1] ZHOU Anbang;XU Yuanyuan;GUO Xiaoying;ZHOU Yu;DAI Pingping;NONG Sijie;WU Biaoliang(Department of Endocrinology,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Graduate School,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院附属医院内分泌科,广西百色533000 [2]右江民族医学院研究生学院,广西百色533000

出　　处：《右江医学》2023年第3期222-225,共4页Chinese Youjiang Medical Journal

基　　金：国家自然科学基金(81960875)。

摘　　要：目的分析美宝湿润烧伤膏(moist exposed burn ointment,MEBO)对高糖培养的成纤维细胞外泌体分泌的影响,探究MEBO促进糖尿病慢性创面愈合的机制。方法首先将Wistar大鼠表皮于无菌环境中分离。培养分离后的成纤维细胞,等到第四代,根据课题组前期研究将细胞分成四组,分别为对照组(高糖培养基)、MEBO低浓度组(20 g/L)、MEBO中浓度组(40 g/L)、MEBO高浓度组(80 g/L)。药物干预3天后,收集细胞上清用超滤管浓缩后,用SBI试剂盒提取外泌体,采用Western blotting检测外泌体标记蛋白CD81、TSG101。结果外泌体标记蛋白CD81、TSG101在MEBO组和对照组中均有表达。MEBO各浓度组外泌体分泌量均高于对照组,差异有统计学意义(P<0.05),MEBO中浓度组(40 g/L)分泌外泌体最多,与MEBO低浓度组(20 g/L)、MEBO高浓度组(80 g/L)相比差异均有统计学意义(P<0.01)。结论MEBO可以促进高糖培养的成纤维细胞分泌外泌体。Objective To explore the effect of moist exposed burn ointment(MEBO)on the secretion of exosomes in fibroblasts cultured with high glucose,and to explore the mechanism of MEBO in promoting chronic wound healing in diabetes mellitus.Methods Wistar rat epidermis was first isolated in a sterile environment.After culturing the isolated fibroblasts,until the fourth generation,the cells were divided into four groups according to the previous research of the research group,namely,control group(high glucose medium),MEBO low concentration group(20 g/L),MEBO medium concentration group(40 g/L),and MEBO high concentration group(80 g/L).After 3 days of drug intervention,the supernatant of the collected cells was concentrated by ultrafiltration tubes,and the exosomes were extracted by an SBI kit,and exosomes marker proteins CD81 and TSG101 were detected by Western blotting.Results Exocrine marker proteins CD81 and TSG101 were expressed in both the MEBO group and the control group.Exosome secretions in all concentration groups of MEBO were higher than that in the control group,and difference was statistically significant(P<0.05),the MEBO medium concentration group(40 g/L)secreted the most exosomes,while comparing with the MEBO low concentration group(20 g/L)and the MEBO high concentration group(80 g/L),the differences were all statistically significant(P<0.01).Conclusion MEBO can promote the secretion of exosomes from fibroblasts cultured in high glucose.

关 键 词：湿润烧伤膏 糖尿病 外泌体 成纤维细胞 

分 类 号：R587.2[医药卫生—内分泌]