最短尾短体线虫转录组及潜在效应蛋白分析  

Transcriptome and Candidate Effectors Analysis of Pratylenchus brachyurus

作　　者：扈丽丽 林柏荣[2,3] 王宏洪[4] 陈建松 廖金铃 卓侃 HU Li-li;LIN Bo-rong;WANG Hong-hong;CHEN Jian-song;LIAO Jin-ling;ZHUO Kan(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangdong Academy of Forestry,Guangzhou 510520;Laboratory of Plant Nematology,South China Agricultural University,Guangzhou 510642;Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Guangzhou 510642;Guangdong Eco-Engineering Polytechnic,Guangzhou 510520)

机构地区：[1]广东省森林培育与保护利用重点实验室,广东省林业科学研究院,广州510520 [2]华南农业大学植物线虫研究室,广州510642 [3]广东省微生物信号与病害防治重点实验室,广州510642 [4]广东生态工程职业学院,广州510520

出　　处：《生物技术通报》2023年第3期254-266,共13页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31401716,31772136,31672007)。

摘　　要：最短尾短体线虫(Pratylenchus brachyurus)是一种重要的迁移性内寄生植物病原线虫。对其转录组进行测序和分析,是进一步研究其寄生特点和致病机理的重要基础。通过转录组测序和分析,得到72516个unigenes,其中38266个(52.76%)unigenes注释到7个蛋白质数据库中。Cazyme分析预测到541条具有植物/真菌细胞壁降解活性的蛋白质序列,分别属于GH5、GH30、GH53、GH28、PL3、GH16、GH18和GH20家族。转录组中有56个蛋白序列与秀丽隐杆线虫(Caenorhabditis elegans)的RNAi通路蛋白具有同源性。鉴定到51个与已知植物寄生线虫效应蛋白具有同源性的蛋白序列,检测了9个潜在效应蛋白在抑制植物防卫反应中的作用,其中7个能够抑制由Gpa2/Rbp-1引起的细胞程序性死亡反应。研究结果表明最短尾短体线虫转录组与同属的短体线虫基因分布特点相似,其潜在效应蛋白具有抑制植物防卫反应的特点。Pratylenchus brachyurus is an important migratory endoparasitic phytopathogenic nematode.Analysis of the transcriptome is an important basis for further research on its parasitic characteristics and pathogenic mechanism.In the transcriptome of P.brachyurus,72 516 unigenes were generated and 38 266(52.76%)unigenes were annotated against seven protein databases.In the carbohydrate-active enzymes analysis,541 proteins were predicted to have plant/fungal cell wall degrading activities,and belonged to the families of GH5,GH30,GH53,GH28,PL3,GH16,GH18 and GH20 respectively.There were 56 protein sequences in the transcriptome demonstrating similarities with orthologues involved in RNAi pathway of Caenorhabditis elegans.Further analysis showed that 51 protein sequences with homology to known plant-parasitic nematode effector proteins were identified.Nine candidate effectors were selected and their potential ability in suppressing plant defense was tested.Seven of them suppressed programmed cell death(PCD)triggered by Gpa2/Rbp-1.The results revealed that the gene distribution characteristics of P.brachyurus were similar to of the same genus of Pratylenchus spp.The candidate effectors may play a critical role in suppressing plant defense.

关 键 词：最短尾短体线虫 转录组 效应蛋白 细胞程序性死亡 

分 类 号：S432.45[农业科学—植物病理学]