新型异养硝化-好氧反硝化菌Paracoccus sp.QD-19的分离及脱氮特性研究  

Isolation of a Novel Heterotrophic Nitrification-Aerobic Denitrification Bacterium Paracoccus sp.QD-19 and Its Characterization of Removing Nitrogen

作　　者：张宇红 董先博 刘香宇 许家琪 徐子祾 ZHANG Yu-hong;DONG Xian-bo;LIU Xiang-yu;XU Jia-qi;XU Zi-ling(College of Environmental and Safety Engineering,Shenyang University of Chemical Technology,Shenyang 110142)

机构地区：[1]沈阳化工大学环境与安全工程学院,沈阳110142

出　　处：《生物技术通报》2023年第3期301-310,共10页Biotechnology Bulletin

基　　金：辽宁省教育厅面上项目(LJKZ0471);大学生创新训练计划项目(X202110149097)。

摘　　要：从沈阳市南部污水处理厂活性污泥中分离获得同时具备异养硝化和好氧反硝化能力的新型菌株,研究其脱氮特性,为改善污水厂的脱氮处理工艺奠定基础。对菌株进行形态学观察和16S rRNA基因鉴定;分别以NH_(4)Cl、NaNO_(2)、KNO_(3)为唯一氮源探究菌株的脱氮能力;以碳源、C/N比、pH值、温度、转速、接种量(V∶V)等因素对菌株脱氮效果的影响进行研究。获得一株新型异养硝化-好氧反硝化菌株,经16S rRNA基因序列比对为副球菌属(Paracoccus),命名为Paracoccus sp.QD-19。菌株对初始氨氮浓度在300 mg/L以下的低浓度氨氮去除率能够达到100%,去除速率为8.707 mg/(L·h)且在脱氮过程中几乎没有亚硝态氮和硝态氮的积累。以亚硝态氮和硝态氮作为唯一氮源时,对此两种氮源的去除率36 h内均能达到99%,去除速率分别为4.944和5.666mg/(L·h)。确定了去除氨氮的最佳脱氮条件:琥珀酸钠为碳源,C/N比为10,pH值为7,接种量(V:V)为1%,温度为30℃,转速为140 r/min。菌株Paracoccus sp.QD-19同时具有异养硝化和好氧反硝化的能力,并且具备不积累亚硝态氮和硝态氮的特性,为污水厂脱氮工艺的改进提供了实践基础。The study is aimed to isolate a novel strain with simultaneous heterotrophic nitrification and aerobic denitrification ability from the activated sludge of the south sewage treatment plant in Shenyang,and to evaluate the nitrogen-removing characteristics,which may lay a foundation for improving nitrogen removal process in wastewater facility.The strain Paracoccus sp.QD-19 was observed by morphological and identified by 16S rRNA gene sequencing.The nitrogen-removing capabilities of the strain were explored by NH_4Cl,NaNO_2,and KNO_3 as sole nitrogen source.The effects of carbon source types,C/N ratio,pH value,temperature,shaking speed and inoculation amount(v:v)on the nitrogen removal were explored.A novel strain with heterotrophic nitrification-aerobic denitrification was obtained,identified as Paracoccus by 16S rRNA gene sequencing,and designated as that Paracoccus sp.QD-19.Results showed that 100% of ammonia nitrogen(below 300 mg/L)in wastewater was removed by the strain,and the removal rates achieved 8.707 mg/(L·h).There was no nitrite accumulation in the nitrogen removal process.The stain Paracoccus sp.QD-19 removed 99% of nitrite or nitrate nitrogen in wastewater in 36 h,and the removal rates achieved 4.944 and 5.666 mg/(L·h)separately.The optimal conditions for ammonia nitrogen removal were:sodium succinate as carbon resource,10(C/N ratio),7(pH value),1%(inoculation amount),30℃(temperature),and 140 r/min(shaking speed).These findings indicate that the strain Paracoccus sp.QD-19 possesses heterotrophic nitrification-aerobic denitrification capability,and the characterization without the accumulation of nitrite and nitrate nitrogen,which provides the practical basis for the improvement of nitrogen-removing process in the wastewater treatment plant.

关 键 词：生物脱氮 异养硝化 同步硝化反硝化 副球菌 污水处理 

分 类 号：X703[环境科学与工程—环境工程] X172