First molecular identification of multiple tick‑borne pathogens in livestock within Kassena‑Nankana,Ghana  

作　　者：Seth Offei Addo Ronald Essah Bentil Kevin Nii Yartey Jane Ansah‑Owusu Eric Behene Selassie Bruku Victor Asoala Suzanne Mate John Asiedu Larbi Philip Kweku Baidoo Michael David Wilson Joseph W.Diclaro II Samuel K.Dadzie 

机构地区：[1]Parasitology Department,Noguchi Memorial Institute for Medical Research,College of Health Sciences,University of Ghana,Accra,Ghana [2]Department of Theoretical and Applied Biology,College of Science,KNUST,Kumasi,Ghana [3]Department of Epidemiology,Noguchi Memorial Institute for Medical Research,College of Health Sciences,University of Ghana,Accra,Ghana [4]Navrongo Health Research Centre,Upper East Region,Navrongo,Ghana [5]U.S.Army Emerging Infectious Diseases Branch,Walter Reed Army Institute of Research,Silver Spring,MD,USA [6]Navy and Marine Corps Public Health Center,Center for Disease Control and Prevention Detachment,Atlanta,GA,USA

出　　处：《Animal Diseases》2023年第1期21-34,共14页动物疾病（英文）

基　　金：funded by the Uniformed Services University Center for Global Health Engagement(CGHE)through the Global Health Engagement Research Initiative(Grant number:GRANT12767296).

摘　　要：The risk of pathogen transmission continues to increase significantly in the presence of tick vectors due to the trade of livestock across countries.In Ghana,there is a lack of data on the incidence of tick-borne pathogens that are of zoonotic and veterinary importance.This study,therefore,aimed to determine the prevalence of such pathogens in livestock using molecular approaches.A total of 276 dry blood spots were collected from cattle(100),sheep(95)and goats(81)in the Kassena-Nankana Districts.The samples were analyzed using Polymerase Chain Reaction(qPCR)and conventional assays and Sanger sequencing that targeted pathogens including Rickettsia,Coxiella,Babesia,Theileria,Ehrlichia and Anaplasma.An overall prevalence of 36.96%was recorded from the livestock DBS,with mixed infections seen in 7.97%samples.Furthermore,the prevalence of infections in livestock was recorded to be 19.21%in sheep,14.13%in cattle,and 3.62%in goats.The pathogens identified were Rickettsia spp.(3.26%),Babesia sp.Lintan(8.70%),Theileria orientalis(2.17%),Theileria parva(0.36%),Anaplasma capra(18.48%),Anaplasma phagocytophilum(1.81%),Anaplasma marginale(3.26%)and Anaplasma ovis(7.25%).This study reports the first molecular identification of the above-mentioned pathogens in livestock in Ghana and highlights the use of dry blood spots in resource-limited settings.In addition,this research provides an update on tick-borne pathogens in Ghana,suggesting risks to livestock production and human health.Further studies will be essential to establish the distribution and epidemiology of these pathogens in Ghana.

关 键 词：LIVESTOCK RICKETTSIA BABESIA THEILERIA EHRLICHIA ANAPLASMA 

分 类 号：S82[农业科学—畜牧学]