Host defense peptide-mimickingβ-peptide polymer displaying strong antibacterial activity against cariogenic Streptococcus mutans  

作　　者：Yi Yang Yuxin Qian Mingxing Zhang Shuang Hao Hui Wang Yongqiang Fan Runhui Liu Dake Xu Fuhui Wang 

机构地区：[1]Life and Health Sciences,Northeastern University,Shenyang 110819,China [2]Shenyang National Laboratory for Materials Science,Northeastern University,Shenyang 110819,China [3]Electrobiomaterials Institute,Key Laboratory for Anisotropy and Texture of Materials,Ministry of Education,Northeastern University,Shenyang 110819,China [4]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China [5]School of Environmental and Safety Engineering,Liaoning Petrochemical University,Fushun 113001,China [6]Key Laboratory for Ultrafine Materials of Ministry of Education,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Research Center for Biomedical Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai 200237,China

出　　处：《Journal of Materials Science & Technology》2023年第2期77-88,共12页材料科学技术（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.51871050);the National Natural Science Foundation of China(No.U2106206);the Natural Science Foundation of Liaoning Province(No.20180510041);the Liaon-ing Revitalization Talents Program(No.XLYC1907158);the Gen-eral Project of Natural Science Foundation of Science and Tech-nology Department of Liaoning Province(No.2021-MS-308);the Fundamental Research Funds for the Central Universities(No.N2120007).

摘　　要：Cariogenic Streptococcus mutans(S.mutans)is a leading cause of bacterial-induced oral diseases.Current strategies to kill bacteria based on Host defense peptide(HDP)mimicking polymers hold promise to treat oral bacterial infection.Here,we explore the impact of hydrophobic subunit and chain length variation on the antibacterial and antibiofilm activity ofβ-peptide polymers.The physicochemical and biological prop-erties,such as the toxicity,the antibacterial activity,and the effect on bacterial transcription ofβ-peptide polymers,were systematically investigated with numerous techniques.The results exhibited that the op-timalβ-peptide polymer has low toxicity towards human periodontal ligament fibroblasts,andβ-peptide polymers(especially P3)have more excellent antibacterial activity against S.mutans than metronidazole.In addition,β-peptide polymers inhibited the reversible and irreversible bacterial adhesion during the formation of biofilms.The polymer can promote biofilm dispersion by decreasing the hydrophobicity of bacterial cells after adhering to cell surfaces.Analysis of the transcriptome for S.mutans treated withβ-peptide polymers demonstrated thatβ-peptide polymers could reduce the cariogenicity of S.mutans by impacting the transcription of the energy and acid metabolism-related genes.β-peptide polymers are promising antimicrobial agents in clinical dentistry due to their high antibacterial efficiency and low tox-icity.

关 键 词：β-peptide polymers Host defense peptide S.mutans RT-QPCR TRANSCRIPTOME 

分 类 号：TQ317[化学工程—高聚物工业]