郑州市非伤寒沙门菌血清型和耐药性分析  

Serotypes and drug resistance of non-typhoidal Salmonella in Zhengzhou City

作　　者：赵雪蕾 周鹏 安戈[1] 李羿 张晓甍 程春荣[1] ZHAO Xuelei;ZHOU Peng;AN Ge;LI Yi;ZHANG Xiaomeng;CHENG Chunrong(Microbiological Laboratory,Zhengzhou Center for Disease Control and Prevention,Zhengzhou,Henan 450007,China)

机构地区：[1]郑州市疾病预防控制中心微生物检验所,河南郑州450007

出　　处：《预防医学》2023年第4期335-338,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：河南省细菌性传染病病原谱流行规律及变异研究(2018ZX10713003-002-003)。

摘　　要：目的了解郑州市非伤寒沙门菌的血清型和耐药情况,为预防和控制非伤寒沙门菌感染提供依据。方法收集2017—2021年郑州市哨点医院腹泻病例分离的非伤寒沙门菌,采用玻片凝集法和软琼脂诱导法进行血清分型,采用微量肉汤稀释法进行药敏试验。结果446株非伤寒沙门菌分离鉴定出5种血清群和37种血清型,优势血清型为肠炎沙门菌和鼠伤寒沙门菌,分别为210和133株,占47.09%和29.82%。非伤寒沙门菌对氨苄西林、氨苄西林/舒巴坦、萘啶酸、四环素和多西环素的耐药率较高,分别为79.60%、58.74%、56.05%、54.26%和54.04%。多重耐药菌290株,多重耐药率为65.02%;其中肠炎沙门菌和鼠伤寒沙门菌多重耐药率分别为70.48%和67.67%。结论2017—2021年郑州市腹泻病例非伤寒沙门菌的血清型分布广泛,肠炎沙门菌和鼠伤寒沙门菌为优势血清型;非伤寒沙门菌普遍耐药且存在多重耐药现象。Objective To investigate the serotypes and drug resistance of non-typhoidal Salmonella in Zhengzhou City,so as to provide insights into prevention and control of non-typhoidal Salmonella infections.Methods Salmonella iso⁃lates were collected from diarrheal patients in Zhengzhou municipal sentinel hospitals from 2017 to 2021.Salmonella se⁃rotypes were identified using slide agglutination test and soft agar colony formation assay,and antimicrobial susceptibili⁃ty test was performed using the broth micro-dilution method.Results Five serogroups and 37 serotypes were identified among 446 non-typhoidal Salmonella isolates,with S.enteritidis(210 isolates,47.09%)and S.typhimurium(133 iso⁃lates,29.82%)as dominant serotypes.Non-typhoidal Salmonella showed high resistance to ampicillin(79.60%),ampicil⁃lin/sulbactam(58.74%),naphthyric acid(56.05%),tetracycline(54.26%)and doxycycline(54.04%),respectively.There were 290 multidrug-resistant Salmonella isolates(65.02%),and the multidrug resistance rates were 70.48%for S.enterit⁃idis and 67.67%for S.typhimurium,respectively.Conclusions Multiple serotypes of non-typhoidal Salmonella were identified in Zhengzhou City from 2017 to 2021,with S.enteritidis and S.typhimurium as dominant serotypes.Wide⁃spread drug resistance and multidrug resistance was seen in non-typhoidal Salmonella.

关 键 词：沙门菌 血清型 耐药 

分 类 号：R378.22[医药卫生—病原生物学]