电针对小鼠海马C3/C3aR/STAT3信号通路的影响  

Effects of Electroacupuncture on Hippocampal C3/C3aR/STAT3 Signaling Pathway in Mice

作　　者：陈丽敏 郭婉清 温若兰 郑雪花[3] 林岚[3] 王丰[3] 董卫国[3] CHEN Limin;GUO Wanqing;WEN Ruolan;ZHENG Xuehua;LIN Lan;WANG Feng;DONG Weiguo(College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;The Third Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China;College of Integrated Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学针灸学院,福建福州350122 [2]福建中医药大学附属第三人民医院,福建福州350108 [3]福建中医药大学中西医结合学院,福建福州350122

出　　处：《中国中医药信息杂志》2023年第4期71-76,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81973923);福建省中医药科研项目(2021zyjc23)。

摘　　要：目的 观察电针对快速老化模型小鼠SAMP8海马突触功能及C3/C3aR/STAT3信号通路的影响,从补体角度探讨电针改善阿尔茨海默病(AD)突触功能障碍的机制。方法 24只SAMP8小鼠随机分为模型组12只和电针组12只,以12只同月龄抗快速老化小鼠SAMR1作为对照组。电针组选取“百会”“大椎”“肾俞”,每日电针20 min,8 d为1个疗程,每个疗程间隔2 d,共3个疗程。模型组及对照组不予干预。Morris水迷宫实验检测小鼠学习记忆能力;免疫荧光染色检测海马CA1区星形胶质细胞标记物胶质纤维酸性蛋白(GFAP)与补体3(C3)共定位情况和小胶质细胞标记物离子钙结合衔接分子1(Iba1)与补体3a受体(C3aR)共定位情况,并计算共定位阳性细胞数;免疫印迹法检测海马组织突触素(Syn)、突触后致密物95(PSD-95)及C3、C3aR、信号传导与转录激活因子3(STAT3)、p-STAT3蛋白表达;实时荧光定量PCR检测海马组织C3、C3aR和STAT3 mRNA水平。结果 与对照组比较,模型组小鼠Morris水迷宫实验逃避潜伏期明显延长,穿越平台次数及原平台象限停留时间明显减少(P<0.01),海马CA1区GFAP与C3共定位、Iba1与C3aR共定位阳性细胞数明显增多(P<0.01),海马组织Syn、PSD-95蛋白表达明显降低(P<0.01),C3、C3aR、STAT3、p-STAT3蛋白及C3、C3aR、STAT3 mRNA表达明显升高(P<0.01);与模型组比较,电针组小鼠Morris水迷宫实验逃避潜伏期明显缩短,穿越平台次数及原平台象限停留时间明显增加(P<0.05,P<0.01),海马CA1区GFAP与C3共定位、Iba1与C3aR共定位阳性细胞数明显减少(P<0.01),海马组织Syn、PSD-95蛋白表达明显升高(P<0.01),C3、C3aR、STAT3、p-STAT3蛋白及C3、C3aR、STAT3 mRNA表达明显降低(P<0.05,P<0.01)。结论 电针可改善SAMP8小鼠突触功能,提高小鼠学习记忆能力,其机制可能与抑制海马C3/C3aR/STAT3信号通路有关。Objective To observe the effects of electroacupuncture on synaptic function and C3/C3aR/STAT3signaling pathway in hippocampus of SAMP8 mice;To explore the mechanism of electroacupuncture in improving Alzheimer disease(AD) synaptic dysfunction from the perspective of complement.Methods Totally 24 SAMP8mice were divided into model group(12) and electroacupuncture group(12),and 12 SAMR1 mice with the same age were set as control group.“Baihui”(DU20),“Dazhui”(DU14) and “Shenshu”(BL23) were selected in the electroacupuncture group,electroacupuncture was performed daily for 20 min,8 days as a course of treatment,and each course of treatment was separated by 2 days,for a total of 3 courses of treatment.The model group and the control group were not intervened.Morris water maze was used to test the learning and memory ability of mice.Immunofluorescence staining was used to detect the co-localization of the astrocyte marker glial fibrillary acidic protein(GFAP) and C3 and the microglia marker ion calcium-binding adaptor 1(Iba1) and C3aR in the hippocampal CA1 region,and the number of colocalized positive cells was calculated.Western blot was used to detect synaptophysin(Syn),postsynaptic dense 95(PSD-95),C3,C3aR,STAT3 and p-STAT3 protein expressions in hippocampus,real-time fluorescence quantitative PCR was used to detect the mRNA levels of C3,C3aR and STAT3in hippocampus.Results Compared with the control group,the escape latency in Morris water maze test increased in the model group,the number of crossing the platform and the stay time of the original platform quadrant were significantly reduced(P<0.01),the number of positive cells co-localized with GFAP and C3 and co-localized with Iba1 and C3aR in hippocampal CA1 region significantly increased(P<0.01),the protein expressions of Syn and PSD-95 in hippocampus significantly decreased(P<0.01),the expression of C3,C3aR,STAT3,p-STAT3 protein and C3,C3aR,STAT3 mRNA significantly increased(P<0.01).Compared with the model group,the escape latency in Morris water maz

关 键 词：阿尔茨海默病 电针 补体 小胶质细胞 星形胶质细胞 小鼠 

分 类 号：R245.3[医药卫生—针灸推拿学]