链脲佐菌素对糖尿病小鼠生精功能和睾丸m6A甲基化酶表达的影响  

Effects of streptozotocin on spermatogenic function and testicular m6A methylase expression in diabetic mice

作　　者：孟星圻 邓靖 彭莉轩 张圆 林祎 周小兵 曹文宇 李素云 Meng Xingqi;Deng Jing;Peng Lixuan;Zhang Yuan;Lin Yi;Zhou Xiaobing;Cao Wenyu;Li Suyun(Hengyang Medical School,University of South China Clinical Anatomy&Reproductive Medicine Application Institute,Hengyang 421001,Hunan Province,China;Hengyang Medical School,University of South China Clinical Medicine,Hengyang 421001,Hunan Province,China;Hengyang Medical School,University of South China Hunan Provincial Key Laboratory of Tumor Cell and Molecular Pathology,Cancer Institute,Hengyang 421001,Hunan Province,China)

机构地区：[1]南华大学衡阳医学院应用解剖学与生殖医学研究所,湖南衡阳421001 [2]南华大学衡阳医学院,湖南衡阳421001 [3]南华大学衡阳医学院肿瘤研究所,肿瘤细胞和分子病理学湖南省重点实验室,湖南衡阳421001

出　　处：《中国临床解剖学杂志》2023年第2期182-186,共5页Chinese Journal of Clinical Anatomy

基　　金：湖南省自然科学基金(2021JJ30593);湖南省教育厅重点基金(19A428);湖南省大学生研究性学习与创新性实验项目(S202010555091);南华大学大学生研究性学习与创新性实验项目(X202010555380)。

摘　　要：目的探讨链脲佐菌素(streptozotocin,STZ)对糖尿病小鼠生精功能和睾丸m6A甲基化酶表达的影响。方法20只8周龄雄性ICR小鼠,随机分为溶媒对照组和模型组,腹腔注射STZ诱导糖尿病动物模型。注射后第2、4、6、8周检测血糖及体重;第8周采用精子计数法检测小鼠精子数量,HE染色检测精子存活率及睾丸形态,称量睾丸、附睾的重量;Real-time PCR和Western blotting检测各组小鼠睾丸中m6A甲基化酶mRNA及蛋白表达。结果与对照组相比,模型组小鼠空腹血糖显著增高,体重降低(P<0.01);睾丸及附睾重量显著下降(P<0.01)、精子数量显著下降(P<0.05);睾丸形态结构破坏,生精小管萎缩,管腔直径变小,各级生精细胞排列紊乱,管腔内精子数量减少;睾丸m6A甲基化酶METTL3、FTO、YTHDF3 mRNA表达降低(P<0.05);METTL3、FTO蛋白质表达显著降低(P<0.05)。结论STZ诱导的糖尿病小鼠出现生精障碍,其生精障碍可能与睾丸内m6A甲基化酶METTL3以及FTO的表达异常有关。Objective To investigate the effects of streptozotocin(STZ)on spermatogenic function and testicular m6A methylase expression in diabetic mice.Methods Twenty 8-week-old male ICR mice were randomly divided into a vehicle control group(NS)and a model group(STZ),and an animal model of diabetes was induced by intraperitoneal injection of STZ.Blood glucose and body weight changes were measured on the 2nd,4th,6th,8th week after STZ injection.On the 8th week,testes and epididymis were weighed and the number of sperm was also counted,HE staining was then used to detect sperm motility and testicular morphology changes.Real-time PCR and Western blotting methods were used to detect the expression level of m6A methylase mRNA and protein in the testis of each group of mice.Results Compared with the NS group,the fasting blood glucose was significantly increased and body weight was significantly decreased in the model group(P<0.01),the weight of the testis and epididymis was significantly decreased(P<0.01),and the number of sperm was significantly decreased(P<0.05);the morphological structure of the testis was destroyed,the seminiferous tubules shrinked,the diameter of the lumen became smaller,the arrangement of spermatogenic cells at all levels was disordered,and the number of sperm in the lumen decreased;the expression of testicular m6A methylases METTL3,FTO,and YTHDF3 mRNA were significantly decreased(P<0.05);the expression of METTL3 and FTO protein were significantly reduced(P<0.05).Conclusions The spermatogenesis disorder in STZ-induced diabetic mice may be related to the abnormal expression of m6A methylase METTL3 and FTO in the testis.

关 键 词：m6A甲基化 链脲佐菌素 糖尿病 生精功能障碍 

分 类 号：R322.64[医药卫生—人体解剖和组织胚胎学]