棉花枯萎病室内苗期抗病性鉴定方法及评价  

Identification and Evaluation of Indoor Resistance to Cotton Fusarium wilt at Seedling Stage

作　　者：龙天宇 邓亚辉 祖倩丽 杨龙 曲延英[1] 陈全家[1] LONG Tianyu;DENG Yahui;ZU Qianli;YANG Long;QU Yanying;CHEN Quanjia(Engineering Research Centre of Cotton,Ministry of Education,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学农学院/棉花教育部工程研究中心,乌鲁木齐830052

出　　处：《新疆农业科学》2023年第2期416-423,共8页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区天山雪松计划(2018XS05);自治区重大科技专项(2020A01002-2)。

摘　　要：【目的】采用优化苗期抗病性鉴定方法及评价,研究陆地棉种质资源对枯萎病尖孢镰刀菌萎蔫专化型(Fusarium oxysporum f.sp.Momordicae)7号生理小种的抗病性,为棉花抗病育种提供基础抗源。【方法】以265份陆地棉为材料,利用伤根灌菌液法于室内进行棉花苗期枯萎病抗性鉴定,以新疆材料军棉1号为感病对照品种,调查记录病级,以相对病情指数作为评价抗性类型指标。【结果】无免疫(I)枯萎病的品种(系),具有高抗(HR)特性的品种(系)5份,抗病(R)特性的品种(系)78份、耐病(T)特性的品种(系)130份、感病(S)特性的品种(系)材料52份,占枯萎病抗性鉴定品种(系)总数的百分比分别为1.9%、29.4%、49.1%和19.6%。【结论】耐枯萎病的品种(系)最多,具抗病性品种(系)次之。【Objective】To determine the resistance of upland cotton germplasm resources to Fusarium oxysporum wilt specific type(Fusarium oxysporumf.sp.Momordicae)race 7,and to optimize the identification method and evaluation of disease resistance at seedling stage,so as to provide basic resistance source for cotton disease resistance breeding.【Methods】Using 265 upland cotton were taken as test materials,and the resistance of cotton to Fusarium wilt at seedling stage was identified by root irrigation method.;Xinjiang Junmian No.1 was used as the susceptible control variety,and the disease grade was recorded by investigation.After that,the relative disease index was used to evaluate the type of resistance.【Results】The Fusarium wilt resistance of 265 upland cotton germplasm resources was evaluated.The results showed that there were 5 varieties(lines)with high resistance to(I),78 varieties(lines)with resistance to(R),130 varieties(lines)resistant to(T)and 52 varieties(lines)with susceptible(S)characteristics,which were not immune to Fusarium wilt,5 varieties(lines)with high resistance to(HR),78 varieties(lines)with high resistance to Fusarium wilt,130 varieties(lines)with disease resistance and 52 varieties(lines)with susceptible(S)characteristics.The percentages in the total number of Fusarium wilt resistance identified varieties(lines)were 1.9%,29.4%,49.1%and 19.6%,respectively.【Conclusion】Through the identification of Fusarium wilt resistance of upland cotton resource materials at seedling stage,it was found that the most resistant varieties(lines)were Fusarium wilt resistant varieties(lines),followed by disease resistant varieties(lines).These findings provide a germplasm resource with definite resistance for cotton breeding for Fusarium wilt resistance in the future.

关 键 词：陆地棉 枯萎病 尖孢镰刀菌 鉴定方法 抗病性评价 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]