缺氧诱导因子和促红细胞生成素及其受体基因在绵羊各组织中表达量  

Studies on Expression of Hypoxia Inducible Factor,Erythropoietin Gene and Its Receptor in Ovine Tissues

作　　者：雷艳 兰斌 余万里 戴小华[1] 蔡鹏 顾伟芳 阿迪莱·艾力 赵红琼[1] LEI Yan;LAN Bin;YU Wanli;DAI Xiaohua;CAI Peng;GU Weifang;Adilai Ali;ZHAO Hongqiong(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2023年第2期485-492,共8页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区天山雪松计划(2020XS06)。

摘　　要：【目的】研究缺氧诱导因子(HIF)及其下游靶基因促红细胞生成素(EPO)及其受体(EPOR)在绵羊正常生理状况下各组织的表达,为靶向调控或缓解绵羊缺氧应激的研究提供组织学依据。【方法】采用实时荧光定量PCR方法,检测4只新疆细毛羊14种组织中HIF、EPO和EPOR基因的相对表达量。【结果】HIF-1α和HIF-2α基因均在绵羊肺组织的相对表达量最高,且极显著高于其它组织(P<0.01);在肺脏HIF-2α基因的表达量约为HIF-1α表达量的5.2倍。EPO基因在绵羊肾脏的相对表达量最高且显著高于垂体、结肠、脾脏、盲肠、肝脏、肾上腺、瘤胃、下丘脑以及心脏(P<0.05)。EPOR基因在肺脏的表达量最高,肺脏和睾丸的相对表达量显著高于脾脏、下丘脑、肾脏、心脏等组织(P<0.05)。【结论】HIF-1α、HIF-2α和EPO及其受体基因在绵羊的肺部或肾脏的高表达,可能是绵羊缺氧应激感受及其调控的重要器官。【Objective】To investigate the expression of hypoxia inducible factor(HIF)and its downstream target gene erythropoietin(EPO)and its receptor(EPOR)in ovine tissues under normal physiological conditions.The results have provided a histological basis for the study of targeted regulation or alleviation of ovine hypoxia stress.【Methods】The relative expression of HIF,EPO and EPOR genes in 14 tissues of 4 Xinjiang fine wool sheep were detected by real-time fluorescence quantitative PCR.【Results】HIF-1αand HIF-2αthe relative expression of genes in ovine lung tissues were the highest and significantly higher than those in other tissues(P<0.01);HIF-2αin lung the gene expression was about HIF-1α5.2 times.The relative expression of EPO gene in ovine kidney was the highest and significantly higher than that in pituitary,colon,spleen,cecum,liver,adrenal gland,rumen,hypothalamus and heart(P<0.05).The expression of EPOR gene in lung was the highest,and the relative expression of lung and testis was significantly higher than that in spleen,hypothalamus,kidney and heart(P<0.05).【Conclusion】HIF-1α,HIF-2αand EPO and its receptor genes are highly expressed in the lung or kidney of sheep,suggesting that these two may be important organs for ovine hypoxia stress perception and regulation.

关 键 词：绵羊 缺氧诱导因子 促红细胞生成素 缺氧应激 

分 类 号：S826[农业科学—畜牧学]