红花均一多糖的结构表征及抑制HepG2增殖活性  

Structural Characterization of a Homogeneous Polysaccharide Isolated from the Flower of Carthamus Tinctorius L.and Its Inhibitory Activity on HepG2 Proliferation

作　　者：徐永斌 冯帅霞 陈洁[1] 龚欢 施松善[1] 王辉俊[1] 王顺春[1] XU Yongbin;FENG Shuaixia;CHEN Jie;GONG Huan;SHI Songshan;WANG Huijun;WANG Shunchun(Institute of Traditional Chinese Medicine,Key Laboratory for Standardization of Chinese Medicines,Ministry of Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学中药研究所,中药标准化教育部重点实验室,上海201203

出　　处：《高等学校化学学报》2023年第4期52-61,共10页Chemical Journal of Chinese Universities

基　　金：国家重大新药创新专项(批准号:2019ZX09735001-004);国家自然科学基金(批准号:82274078)资助。

摘　　要：对红花多糖进行分离提纯和结构表征,探讨了红花均一多糖对HepG2肝癌细胞增殖的抑制作用.采用水提醇沉法提取红花粗多糖;通过阴离子交换色谱柱和分子筛凝胶色谱柱对红花粗多糖进行分离纯化;采用高效凝胶渗透色谱法(HPGPC)对红花多糖的均一性以及分子量分布进行测定;采用单糖组成、甲基化及核磁共振波谱等方法对其进行结构解析.通过体外细胞实验,研究了红花多糖对于肿瘤细胞的作用.从红花中分离得到一种均一多糖(CTPS-1A),通过HPGPC计算得出其分子量为53800.CTPS-1A的单糖组成为n(阿拉伯糖)∶n(半乳糖)=59.2∶40.8.利用甲基化分析其连接方式为T-Galp,1,3-Galp,1,6-Galp,1,3,6-Galp,T-Araf,1,5-Araf和1,3,5-Araf.研究结果表明,CTPS-1A为阿拉伯半乳聚糖(AG),其主链由-β-Galp-(6→1)-β-Galp-(6→组成,并在1,6-β-Galp的3位连有T-β-Galp,1,3-β-Galp,T-α-Araf,1,5-α-Araf,1,3及5-α-Araf等分支.体外培养HepG2细胞实验结果表明,CTPS-1A通过增强细胞核收缩和细胞凋亡对HepG2的生长具有抑制作用.To isolate,purify and characterize the structure of polysaccharide from flowers of Carthamus tinctorius L.,and to investigate the inhibitory effect of its homogeneous polysaccharide on the proliferation of HepG2 hepatoma cells.The polysaccharides in flowers of Carthamus tinctorius L.were extracted by water extraction and alcohol precipi⁃tation.The crude polysaccharides were separated and purified by anion exchange column and molecular sieve gel column.The homogeneity and molecular weight distribution were determined by high performance gel permeation chromatography(HPGPC).Its structure was elucidated by monosaccharide composition,methylation,and nuclear magnetic resonance(NMR)methods.The inhibitory effect of flowers of Carthamus tinctorius L.polysaccharide on tumor cells was studied by in vitro cell experiments.A homogeneous polysaccharide(CTPS-1A)was obtained.The molecular weight of CTPS-1A was 53800.The monosaccharide composition of CTPS-1A was analyzed by gas chroma⁃tography-mass spectrometer(GC-MS)and the monosaccharide composition was n(arabinose)∶n(galactose)=59.2∶40.8.By methylation analysis,the glycosidic linkages were T-Galp,1,3-Galp,1,6-Galp,1,3,6-Galp,T-Araf,1,5-Araf,and 1,3,5-Araf.The results showed that CTPS-1A was an arabinogalactan(AG),its main chain was composed of-β-Galp-(6→1)-β-Galp-(6→,and T-β-Galp,1,3-β-Galp,T-α-Araf,1,5-α-Araf,1,3,5-α-Araf were connected to the 3 position of 1,3,6-Galp.The results of in vitro culture of HepG2 cells showed that CTPS-1A inhibited the growth of HepG2 by enhancing nuclear contraction and apoptosis.

关 键 词：红花 多糖 结构表征 阿拉伯半乳聚糖 HEPG2肝癌细胞 

分 类 号：O629.12[理学—有机化学]