基于DElncRNA-miRNA-mRNA网络化解析藏鸡低氧适应性  

Network Analysis on Hypoxia Adaptability of Tibetan Chicken Based on DElncRNA-miRNA-mRNA

作　　者：张增荣[1,2] 蒋小松 邱莫寒[1] 余春林 杜华锐[1] 李晴云[1] 彭涵[1] 熊霞[1] 宋小燕[1] 胡陈明[1] 夏波[1] 杨礼[1] 陈家磊[1] 杨朝武[1,2] ZHANG Zengrong;JIANG Xiaosong;QIU Mohan;YU Chunlin;DU Huarui;LI Qingyun;PENG Han;XIONG Xia;SONG Xiaoyan;HU Chenming;XIA Bo;YANG Li;CHEN Jialei;YANG Chaowu(Sichuan Animal Science Academy,Chengdu,Sichuan 610066;Animal Breeding and Genetics Key Laboratory of Sichuan Province,Chengdu,Sichuan 610066)

机构地区：[1]四川省畜牧科学研究院,四川成都610066 [2]动物遗传育种四川省重点实验室,四川成都610066

出　　处：《中国家禽》2023年第4期25-29,共5页China Poultry

基　　金：四川省重点研发计划(2021YFYZ0031);四川省科技支撑计划(2021YFYZ0009);四川省财政运行专项(SASA2022CZYX002)。

摘　　要：为了探讨藏鸡低氧适应机制,试验对藏鸡和大恒鸡胚胎心脏组织进行全转录组测序分析,构建了包括5个DElncRNAs、3个miRNAs、19个靶基因参与的ceRNA网络,通过实时荧光定量PCR验证5个候选lncRNAs。结果显示:ENSGALG00000050044、ENSGALG00000008047和ENSGALG00000053982在藏鸡中的表达水平显著低于大恒鸡(P<0.05),获得42条网络化通路与氧化代谢或能量代谢有关。研究表明,藏鸡对低氧的适应不是通过单一途径实现的,可能是通过协调多个miRNAs的表达变化,触发不同的靶基因和代谢途径来实现,结果为研究生物适应低氧条件提供了新的思路。In order to explore the hypoxia adaptation mechanism of Tibetan chicken,the whole transcriptome sequencing of Tibetan chicken and Daheng chicken was carried out for embryonic heart tissues.A ceRNA network including 5 DElncRNAs,3 miRNAs and 19 target genes involved in 42 pathways was constructed and 5 lncRNAs were verified by real-time quantitative polymerase chain reaction.The results showed that the expression levels of ENSGALG00000050044,ENSGALG00000008047 and ENSGALG00000053982 in Tibetan chickens were significantly lower than those in Daheng chicken(P<0.05).Forty-two net⁃worked pathways were obtained,which were related to oxygen metabolism or energy metabolism.The DElncRNA-miR⁃NA-mRNA network map of Tibetan chicken heart tissue was obtained.The results showed that the adaptation of Tibetan chick⁃ens to hypoxia was not realized through a single way,but might be realized by coordinating the expression changes of multiple dif⁃ferent small RNAs and triggering different target genes and metabolic pathways,which would provide a new idea for the study of biological adaptation to hypoxia.

关 键 词：藏鸡 全转录组 网络化图谱构建 低氧适应机制 

分 类 号：S831.2[农业科学—畜牧学]