绞股蓝皂苷对人胃癌细胞增殖和凋亡的影响及作用机制  

Effects of gypenosides on proliferation and apoptosisof human gastric cancer cells and its mechanism

作　　者：邓芙蓉 王翰林 谢佩佩 陈思 张健 DENG Fu-rong;WANG Han-lin;XIE Pei-pei;CHEN Si;ZHANG Jian(College of Pharmacy,Health Center,Shenzhen University,Shenzhen 518060,China;College of Basic Medicine,Health Center,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]深圳大学医学部药学院,广东深圳518060 [2]深圳大学医学部基础医学院,广东深圳518060

出　　处：《中国药理学通报》2023年第4期646-652,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81973531);深圳市自然科学基金资助项目(No 20200812211704001);广东省医学科学技术研究基金(No A2019502)。

摘　　要：目的探索绞股蓝皂苷对人胃癌细胞SGC-7901和AGS增殖和凋亡的影响及其作用机制。方法不同浓度的绞股蓝皂苷分别处理SGC-7901和AGS细胞,采用CCK-8法检测细胞增殖活性并计算得到IC50;细胞克隆实验检测集落形成情况;流式实验检测细胞凋亡;caspase活性检测实验检测细胞内caspase-9和caspase-3的活性;Western blot实验检测细胞内凋亡相关蛋白的表达。结果CCK-8和细胞克隆实验结果显示,绞股蓝皂苷能抑制SGC-7901和AGS细胞的增殖。流式结果表明,绞股蓝皂苷能够促进细胞的凋亡。caspase活性检测结果表明,绞股蓝皂苷能增加细胞内caspase-9和caspase-3的活性。Western blot结果表明,绞股蓝皂苷主要通过上调人胃癌细胞cleaved-PARP、caspase-9和caspase-3蛋白的表达,同时下调Bcl-2蛋白的表达,诱导细胞凋亡。结论绞股蓝皂苷能抑制SGC-7901和AGS细胞的增殖,并调控凋亡途径所涉及的cleaved-PARP、caspase-9、caspase-3和Bcl-2等相关蛋白的表达,诱导细胞凋亡,进而发挥抗肿瘤的作用。Aim To explore the effect of gypenosides on proliferation and apoptosis of human gastric cancer cells SGC-7901 and AGS and its mechanism.Methods Different concentrations of gypenosides were cultured with human gastric cancer cells SGC-7901 and AGS.Cell viability assay was used to detect cell proliferation activity,and the IC 50 of two kinds of cells was calculated.Cell clone formation assay was conducted to evaluate the proliferation inhibition rate.Flow cytometry was applied to detect the apoptosis rate.caspase activity assay was performed to detect the activities of caspase-9 and caspase-3 in cells.The expression of apoptosis-related proteins was analyzed by Western blot in human gastric cancer cells treated by gypenosides.Results Cell viability assay and clone assay demonstrated that the proliferation of gastric cancer cells SGC-7901 and AGS was inhibited by gypenosides.Flow cytometry showed that gypenosides could increase cell apoptosis significantly compared with the control group.caspase activity assay indicated that gypenosides could increase the activities of caspase-9 and caspase-3 in cells.Gypenosides induced apoptosis by up-regulating the expression of cleaved-PARP,caspase-9,caspase-3 and down-regulating the expression of Bcl-2 proteins in gastric cancer cells.Conclusions Gypenosides exert antitumor activity properties by inhibiting the proliferation of SGC-7901 and AGS cells and inducing apoptosis by regulating the expression of related proteins involved in apoptosis pathway,such as cleaved-PARP,caspase-9,caspase-3 and Bcl-2.

关 键 词：绞股蓝皂苷 胃癌 SGC-7901 AGS 细胞增殖 细胞凋亡 凋亡途径 

分 类 号：R284.1[医药卫生—中药学] R329.25[医药卫生—中医学] R329.28R735.2