微滤式微流控系统处理低温保护剂的实验研究  

Microfiltration Microfluidic System for Treatment CPAs of Experimental Study

作　　者：刘杰 周晓明 冷顺 王吉 LIU Jie;ZHOU Xiao-ming;LENG Shun;WANG Ji(School of Mechanical and Electrical Engineering,University of Electronic Science and Technology of China,Sichuan Chengdu 611731,China)

机构地区：[1]电子科技大学机械与电气工程学院,四川成都611731

出　　处：《机械设计与制造》2023年第4期191-195,共5页Machinery Design & Manufacture

基　　金：面向玻璃化保存的微尺度传热机理和强化方法研究(51676030)。

摘　　要：应用微流控技术有望解决于小体积细胞的低温保护剂(CPAs)处理问题,但现有的扩散式和透析式微流控芯片普遍存在传质效率过低的问题。这里提出一种新的膜式微流控系统,通过采用高通性的微滤膜和延长的微流道实现强化的跨膜对流传质。实验结果表明:所设计的芯片在处理过程中产生了自发的“浓缩-稀释”效应,有效地提高了处理效率;含有10%DMSO的溶液在60ml/h速率下单次通过芯片后CPA去除率可达93%,而且在芯片中溶液浓度变化平缓可控,从而提供了一种理想的CPA处理方案。The application of microfluidic technology is expected to solve the problem of cryoprotectant agents(CPAs)treatment of small volume cells,but the existing diffusion and dialysis micrcfluidic chips generally have the problem of low mass transfer efficiency.A new membrane microfluidic system is proposed to achieve enhanced transmembrane convective mass transfer through the use of high-pass microfiltration membranes and extended microchannels.The experimental results show that the designed chip has a spontaneous concentration-dilutionf effect during the processing,which effectively improves the processing efficiency x the CPA removal rate after a single pass of the solution containing 10%DMSO at 60ml/h rate Up to 93%,and the concentration of the solution in the chip changes gently and controllabfy,thus providing an ideal CPA treatment scheme.

关 键 词：微流控 低温保护剂 传质效率 浓缩—稀释 去除率 

分 类 号：TH16[机械工程—机械制造及自动化] TQ320[化学工程—合成树脂塑料工业] Q81[生物学—生物工程]