miR-33a基于Notch信号通路对幼年哮喘小鼠Th17/Treg细胞平衡的影响  

Effect of miR-33a on Th17/Treg cell balance in juvenile asthma mice based on Notch signal pathway

作　　者：程静 年瑞[1] 李万军[1] CHENG Jing;NIAN Rui;LI Wanjun(Department of Pathology,3201 Affiliated Hospital of Medical College of Xi􀆳an Jiaotong University,Hanzhong 723000,Shaanxi,China)

机构地区：[1]西安交通大学医学院附属3201医院病理科,陕西汉中723000

出　　处：《中南医学科学杂志》2023年第2期202-205,225,共5页Medical Science Journal of Central South China

基　　金：2022年3201医院科研基金项目(3201yk202247)。

摘　　要：目的 探究miR-33a通过调控Notch信号通路对幼年哮喘小鼠辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡的影响。方法 小鼠随机分为对照组、哮喘组和miR-33a组(哮喘+miR-33a)。qRT-PCR检测各组肺组织miR-33a水平。比较各组大鼠气道阻力、Th17/Treg细胞平衡及其细胞因子、淋巴细胞Notch信号通路的水平。结果 与对照组比较,哮喘组肺组织miR-33a降低,气道阻力和肺组织损伤程度升高,Th17、Th17/Treg、白细胞介素-17及Notch信号通路水平升高,Treg水平降低(P<0.05);而与哮喘组比较,miR-33a组上述趋势均得到逆转(P<0.05)。结论 miR-33a可通过抑制Notch信号通路,使Th17/Treg平衡向Treg偏移,从而改善哮喘小鼠气道反应性。Aim To explore the effect of miR-33a on the balance of helper T cell 17(Th17)/regulatory T cell(Treg)in juvenile asthma mice by regulating the Notch pathway.Methods The mice were randomly divided into control group,asthma group and miR-33a group(asthma+miR-33a).The level of miR-33a in lung tissue of each group was detected by qRT-PCR.Compare the levels of airway resistance,the levels of airway resistance,Th17/Treg cell balance,cytokines and Notch pathway in lymphocytes were compared in each group.Results Compared with the control group,the lung tissue miR-33a in the asthma group decreased,the airway resistance and the degree of lung tissue injury increased,the levels of Th17,Th17/Treg,interleukin-17 and Notch pathway increased,and the level of Treg decreased(P<0.05).Compared with the asthma group,the above trends in the miR-33a group were reversed(P<0.05).Conclusion miR-33a can shift Th17/Treg balance to Treg by inhibiting Notch pathway,thus improving airway responsiveness of asthma mice.

关 键 词：miR-33a 哮喘 TH17/TREG NOTCH信号通路 

分 类 号：R56[医药卫生—呼吸系统]